Efficacy and mechanism of carvacrol with octanoic acid against mastitis causing multi-drug-resistant pathogens

被引:0
作者
Sapna Rani
Hemlata Singh
Chand Ram
机构
[1] ICAR-National Dairy Research Institute (Deemed University),Synbiotic Functional Food and Bioremediation Research Laboratory, Dairy Microbiology Division
来源
Brazilian Journal of Microbiology | 2022年 / 53卷
关键词
Antimicrobial mechanism; Food safety; Mastitis; Multi-drug-resistant pathogens; Natural bioactive components;
D O I
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中图分类号
学科分类号
摘要
In the present investigation, we determined the in vitro antimicrobial activity of eight essential oils (EOs) and three medium-chain fatty acids (MCFAs) alone and in combination against Staphylococcus aureus ATCC 700698, Klebsiella pneumoniae ATCC 700603, and E. coli FcW5. The interactions between EOs and MCFAs were determined by fractional inhibitory concentration indices. Moreover, mode of action of selected bioactive components was studied by changes in bacterial surface charge, morphology, and membrane integrity assays. Among EOs, carvacrol (CAR), trans-cinnamaldehyde (TC), and thymol (TM) showed strong antimicrobial activity. In combination study, CAR+OA (octanoic acid), CAR+DA (decanoic acid), and TM+OA were observed as the most significant (P≤0.05) which were also confirmed through time-kill plots. Based on these results, CAR+OA were found to be most efficacious in terms of killing time (P≤0.05). Changes in the surface charge, morphology, and membrane integrity upon the combined treatment of CAR+OA were also observed, which ultimately leads to cell death. Results suggest that CAR+OA when used in combination offer a significant (P≤0.05) additive antimicrobial activity against the selected pathogenic bacteria. Therefore, these natural bioactive molecules could be interesting alternatives to conventional therapy for the control of mastitis caused by multi-drug-resistant pathogens in bovine animals to ensure the milk safety.
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页码:385 / 399
页数:14
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