Direct and indirect shoot and bulblet regeneration from cultured leaf explants of Lilium pumilum, an endangered species

被引:0
作者
Shumei Jin
Ji Wang
Xinwang Wang
Dan Sun
Guoliang Li
A. D. Genovesi
Shengkui Liu
机构
[1] Northeast Forestry University,Key Laboratory of Saline
[2] Texas A&M University System,Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC)
[3] Maize Research Institute of Heilongjiang Academy of Agricultural Sciences,Texas A&M AgriLife Research Center
来源
In Vitro Cellular & Developmental Biology - Plant | 2014年 / 50卷
关键词
Callus; Differentiation; regeneration; Leaf explants; Lily;
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中图分类号
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摘要
Alternative methods of in vitro cloning that involve both adventitious (direct) and callus intermediate (indirect) pathways were investigated for the endangered species Lilium pumilum. Plantlet regeneration was obtained from leaf explants, cultured on Murashige and Skoog (MS) basal medium supplemented with various combinations of auxins and cytokinins at different concentrations. About 30% of the explants directly formed adventitious shoots on MS medium containing 8.88 μM 6-benzyladenine (BA) and 2.69 μM α-naphthaleneacetic acid (NAA). For production of regenerable callus, callus formation followed by shoot induction was best when explants were initially cultured on MS medium supplemented with 9.05 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Regenerable calli were yellow or purple and readily regenerated shoots when subcultured onto MS medium containing 2.22 μM BA and 1.61 μM NAA. About 78% of the calli were able to produce adventitious shoots. Shoots were rooted on half-strength MS medium supplemented with 1.34 μM NAA and were successfully acclimatized to greenhouse conditions. This report describes an efficient method for the in vitro multiplication of whole plants from leaf explants of the endangered species L. pumilum.
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页码:69 / 75
页数:6
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