The synthetic opioid fentanyl increases HIV replication and chemokine co-receptor expression in vitro

被引:0
作者
Ling Kong
Mohamed Tarek M. Shata
Jennifer L. Brown
Michael S. Lyons
Kenneth E. Sherman
Jason T. Blackard
机构
[1] University of Cincinnati College of Medicine,Division of Digestive Diseases, Department of Internal Medicine
[2] University of Cincinnati College of Medicine,Addiction Sciences Division, Department of Psychiatry and Behavioral Neuroscience
[3] University of Cincinnati,Department of Psychology
[4] University of Cincinnati College of Medicine,Department of Emergency Medicine
[5] University of Cincinnati College of Medicine,Center for Addiction Research
来源
Journal of NeuroVirology | 2022年 / 28卷
关键词
Opioid; Fentanyl; Drug use; Chemokine; HIV;
D O I
暂无
中图分类号
学科分类号
摘要
The US is experiencing a major public health crisis that is fueled by the illicit use of synthetic opioids including fentanyl. While several drugs of abuse can enhance viral replication and/or antagonize immune responses, the impact of specific synthetic opioids on HIV pathogenesis is poorly understood. Thus, we evaluated the effects of fentanyl on HIV replication in vitro. HIV-susceptible or HIV-expressing cell lines were incubated with fentanyl. HIV p24 synthesis and chemokine receptor levels were quantified by ELISA in culture supernatants and cell lysates, respectively. Addition of fentanyl resulted in a dose-dependent increase in HIV replication. Fentanyl enhanced expression of the HIV chemokine co-receptors CXCR4 and CCR5 and caused a dose-dependent decrease in cell viability. The opioid antagonist naltrexone blocked the effect of fentanyl on HIV replication and CCR5 receptor levels but not CXCR4 receptor levels. TLR9 expression was induced by HIV; however, fentanyl inhibited TLR9 expression in a dose-dependent manner. These data demonstrate that the synthetic opioid fentanyl can promote HIV replication in vitro. As increased HIV levels are associated with accelerated disease progression and higher likelihood of transmission, additional research is required to enhance the understanding of opioid-virus interactions and to develop new and/or optimized treatment strategies for persons with HIV and opioid use disorder.
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页码:583 / 594
页数:11
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