A microarray analysis of wheat grain hardness

被引：0
作者：
Bryan Clarke
Sadequr Rahman
机构：
[1] CSIRO Division of Plant Industry,
[2] NSWCAG Division of Plant Industry,undefined
来源：
Theoretical and Applied Genetics | 2005年 / 110卷
关键词：
Differential Gene Expression; Northern Analysis; Endosperm Development; Grain Texture; Soft Wheat;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Grain hardness is an important quality characteristic of wheat grain, and considerable research effort has focused on characterising the genetic and biochemical basis underlying the hardness phenotype. Previous research has shown that the predominant difference between hard and soft seeds is linked to the puroindoline (PIN) proteins. In this study the near-isogenic lines of Heron and Falcon, which differ only in the grain hardness character, were compared using a cDNA microarray consisting of approximately 5,000 unique cDNA clones that were isolated from wheat and barley endosperm tissue. Our analysis showed that major differences in gene expression were evident for puroindoline-a (Pina), with a minor but not consistent change in the expression of puroindoline-b (Pinb). These observations were confirmed using a 16,000 unique cDNA microarray in a comparison of hard wheats with either the Pina null or Pinb mutation.
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页码：1259 / 1267
页数：8
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