Methods for RNA detection in live cells [Methoden zur RNA-Detektion in lebenden Zellen]

被引：0
|
作者：
Stoddart M.J. [1 ]
Weldon D. [2 ]
机构：
[1] AO Research Institute Davos, Clavadelerstraße 8, Davos
[2] EMD Millipore, 28820 Single Oak Drive, Temecula, 92590, CA
来源：
BIOspektrum | 2015年 / 21卷 / 2期
关键词：
D O I：
10.1007/s12268-015-0560-4
中图分类号：
学科分类号：
摘要：
RNA plays a key regulatory role in cell and tissue development and disease progression. Various detection methods are available to study RNA, but many require cell lysis, which causes significant information loss. This article will describe different RNA detection methods and highlight the challenges and benefits of detecting RNA in live cells. It will then describe a method that allows for live-cell RNA detection within individual cells, illustrating its use in current research. © 2015, Springer-Verlag Berlin Heidelberg.
引用
收藏
页码：191 / 193
页数：2
相关论文
共 50 条
  • [31] An Overview of Current Detection Methods for RNA Methylation
    Saglam, Buket
    Akgul, Bunyamin
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (06)
  • [32] Small RNA Detection by in Situ Hybridization Methods
    Urbanek, Martyna O.
    Nawrocka, Anna U.
    Krzyzosiak, Wlodzimierz J.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (06): : 13259 - 13286
  • [33] RNA detection in disseminated tumor cells
    Zippelius, A
    Lutterbüse, R
    Riethmüller, G
    Pantel, K
    CLINICAL CHEMISTRY, 2001, 47 (02) : 362 - 363
  • [34] Resonance energy transfer methods of RNA detection
    Kyle A. Cissell
    Eric A. Hunt
    Sapna K. Deo
    Analytical and Bioanalytical Chemistry, 2009, 393
  • [35] Live cell RNA detection aids in the understanding of cellular responses
    Weldon, Don
    Ko, Alex
    Williams, Yuko
    Koong, Victor
    JOURNAL OF IMMUNOLOGY, 2013, 190
  • [36] Embarking on a journey through Micro-RNA and Circular-RNA detection methods
    Fakhr, Zhaleh Asadi
    Zhu, Xinlan
    Wang, Hechen
    Ma, Ruiyang
    Lin, Ziwei
    Shen, Xudan
    Liu, Jiatong
    Zeng, Su
    Cai, Sheng
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2024, 181
  • [37] Computational methods for RNA modification detection from nanopore direct RNA sequencing data
    Furlan, Mattia
    Delgado-Tejedor, Anna
    Mulroney, Logan
    Pelizzola, Mattia
    Novoa, Eva Maria
    Leonardi, Tommaso
    RNA BIOLOGY, 2021, 18 : 31 - 40
  • [38] DNA Transformer for Visualizing Endogenous RNA Dynamics in Live Cells
    Wan, Ying
    Zhu, Ninghao
    Lu, Yi
    Wong, Pak Kin
    ANALYTICAL CHEMISTRY, 2019, 91 (04) : 2626 - 2633
  • [39] Illuminating RNA Dynamics in Live Mammalian Cells with the Riboglow Platform
    Braselmann, Esther
    BIOPHYSICAL JOURNAL, 2021, 120 (03) : 316A - 316A
  • [40] RNA imaging in living cells - Methods and applications
    Urbanek, Martyna O.
    Galka-Marciniak, Paulina
    Olejniczak, Marta
    Krzyzosiak, Wlodzimierz J.
    RNA BIOLOGY, 2014, 11 (08) : 1083 - 1095
12345