Sodium Butyrate Induces Hepatic Differentiation of Mesenchymal Stem Cells in 3D Collagen Scaffolds

被引:0
作者
Saman Rashid
Asmat Salim
Rida -e- Maria Qazi
Tuba Shakil Malick
Kanwal Haneef
机构
[1] University of Karachi,Dr. Zafar H. Zaidi Center for Proteomics
[2] ICCBS,Dr. Panjwani Center for Molecular Medicine and Drug Research
[3] University of Karachi,undefined
来源
Applied Biochemistry and Biotechnology | 2022年 / 194卷
关键词
Histone deacetylase inhibitor; Sodium butyrate; Stem cells; Liver regeneration; Collagen scaffold;
D O I
暂无
中图分类号
学科分类号
摘要
Stem cell-based therapy is considered an attractive tool to overcome the burden of liver diseases. However, efficient hepatic differentiation is still a big challenge for the research community. In this study, we explored a novel method for differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into hepatic-like cells using 3D culture conditions and histone deacetylase inhibitor, sodium butyrate (NaBu). MSCs were characterized by the presence of cell surface markers via immunocytochemistry, flow cytometry, and by their potential for osteogenic, adipogenic, and chondrogenic differentiation. MSCs were treated with 1mM NaBu in 2D and 3D environments for 21 days. The hepatic differentiation was confirmed by qPCR and immunostaining. According to qPCR data, the 3D culture of NaBu-treated MSCs has shown significant upregulation of hepatic gene, CK-18 (P < 0.01), and hepatic proteins, AFP (P < 0.01) and ALB (P < 0.01). In addition, immunocytochemistry analysis showed significant increase (P < 0.05) in the acetylation of histones (H3 and H4) in NaBu-pretreated cells. It can be concluded from the study that NaBu-treated MSCs in 3D culture conditions can induce hepatic differentiation without the use of additional cytokines and growth factors. The method shown in this study represents an improved protocol for hepatic differentiation and could contribute to improvement in future cell-based therapeutics.
引用
收藏
页码:3721 / 3732
页数:11
相关论文
共 133 条
[1]  
Rehm J(2013)Global burden of alcoholic liver diseases Journal of Hepatology 59 160-168
[2]  
Samokhvalov AV(2011)Molecular mechanisms of liver regeneration and protection for treatment of liver dysfunction and diseases Journal of Hepato-Biliary-Pancreatic Sciences 18 13-22
[3]  
Shield KD(2020)The dynamic chromatin architecture of the regenerating liver Cellular and Molecular Gastroenterology and Hepatology 9 121-143
[4]  
Fujiyoshi M(2014)Concise review: Therapeutic potential of mesenchymal stem cells for the treatment of acute liver failure and cirrhosis Stem Cells 32 2818-2823
[5]  
Ozaki M(2017)Efficient programming of human mesenchymal stem cell-derived hepatocytes by epigenetic regulations Gastroenterología y Hepatología 32 261-269
[6]  
Wang AW(2008)Transcriptional profiling of young and old mesenchymal stem cells in response to oxygen deprivation and reparability of the infarcted myocardium Journal of Molecular and Cellular Cardiology 44 582-96
[7]  
Wang YJ(2012)The histone acetyltransferase MOF is a key regulator of the embryonic stem cell core transcriptional network Cell Stem Cell. 11 163-178
[8]  
Zahm AM(2014)HDAC signaling in neuronal development and axon regeneration Current Opinion in Neurobiology 27 118-126
[9]  
Morgan AR(2003)Generation of hepatocyte-like cells from human embryonic stem cells Cell Transplantation 12 1-11
[10]  
Wangensteen KJ(2014)Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential BMC Biotechnology 14 105-576