Ex vivo diffusion-weighted MRI tractography of the Göttingen minipig limbic system

被引:0
作者
Johannes Bech
Dariusz Orlowski
Andreas N. Glud
Tim B. Dyrby
Jens Christian H. Sørensen
Carsten R. Bjarkam
机构
[1] Aarhus University,CENSE, Department of Neurosurgery, Department of Clinical Medicine, Aarhus University Hospital
[2] Copenhagen University Hospital Hvidovre,Danish Research Centre for Magnetic Resonance, Center for Functional and Diagnostic Imaging and Research
[3] Technical University of Denmark,Department of Applied Mathematics and Computer Science
[4] Aalborg University Hospital,Department of Neurosurgery, Department of Clinical Medicine
来源
Brain Structure and Function | 2020年 / 225卷
关键词
Cingulum; Fornix; Mammillothalamic tract; Stria medullaris; Stria terminalis; Sus scrofa;
D O I
暂无
中图分类号
学科分类号
摘要
The limbic system encompasses a collection of brain areas primarily involved in higher cognitive and emotional processing. Altered function in the limbic circuitry may play a major role in various psychiatric disorders. This study aims to provide a high-quality ex vivo diffusion-weighted MRI (DWI) tractographic overview of the Göttingen minipig limbic system pathways, which are currently not well described. This may facilitate future translational large animal studies. The study used previously obtained post-mortem DWI scans in 3 female Göttingen minipigs aging 11–15 months. The tractography performed on the DWI data set was made using a probabilistic algorithm, and regions of interest (ROIs) were defined in accordance with a histological atlas. The investigated pathways included the fornix, mammillothalamic tract, stria terminalis, stria medullaris, habenulo-interpeduncular tract, and cingulum. All the investigated limbic connections could be visualized with a high detail yielding a comprehensive three-dimensional overview, which was emphasized by the inclusion of video material. The minipig limbic system pathways displayed using tractography closely resembled what was previously described in both human studies and neuronal tracing studies from other mammalian species. We encountered well-known inherent methodological challenges of tractography, e.g., partial volume effects and complex white matter regions, which may have contributed to derouted false-positive streamlines and the failure to visualize some of the minor limbic pathway ramifications. This underlines the importance of preexisting anatomical knowledge. Conclusively, we have, for the first time, provided an overview and substantial insight of the Göttingen minipig limbic system.
引用
收藏
页码:1055 / 1071
页数:16
相关论文
共 455 条
[1]  
Aggleton JP(1999)Episodic memory, amnesia, and the hippocampal-anterior thalamic axis Behav Brain Sci 22 425-444
[2]  
Brown MW(1986)The origin, course, and termination of the hippocampothalamic projections in the macaque J Comp Neurol 243 409-421
[3]  
Aggleton JP(2015)The habenula–interpeduncular pathway in nicotine aversion and withdrawal Neuropharmacology 96 213-222
[4]  
Desimone R(1994)MR diffusion tensor spectroscopy and imaging Biophys J 66 259-267
[5]  
Mishkin M(2018)The porcine corticospinal decussation: a combined neuronal tracing and tractography study Brain Res Bull 142 253-262
[6]  
Antolin-Fontes B(2007)Probabilistic diffusion tractography with multiple fibre orientations: What can we gain? NeuroImage 34 144-155
[7]  
Ables JL(2009)MRI-guided stereotaxic targeting in pigs based on a stereotaxic localizer box fitted with an isocentric frame and use of SurgiPlan computer-planning software J Neurosci Methods 183 119-126
[8]  
Gorlich A(2017)The telencephalon of the Gottingen minipig, cytoarchitecture and cortical surface anatomy Brain Struct Funct 22 S9-12
[9]  
Ibanez-Tallon I(2008)Neuromodulation in a minipig MPTP model of Parkinson disease Br J Neurosurg 122 45-48
[10]  
Basser PJ(2017)Exposure of the pig CNS for histological analysis: a manual for decapitation, skull opening, and brain removal J Vis Exp JoVE 37 1724-1737