An effective bioremediation approach for enhanced microbial degradation of the veterinary antibiotic sulfamethazine in an agricultural soil

被引：33

作者：

Hirth N. ^{[1
]}

Topp E. ^{[2
]}

Dörfler U. ^{[1
]}

Stupperich E. ^{[3
]}

Munch J.C. ^{[4
]}

Schroll R. ^{[1
]}

机构：

[1] Research Unit Microbe-Plant Interactions, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Neuherberg

[2] Agriculture and Agri-Food Canada, London Research and Development Centre, London, N5V 4T3, ON

[3] Institute of Microbiology and Biotechnology, Ulm University, Ulm

[4] Lehrstuhl für Grünlandlehre, Technische Universität München, Freising

来源：

Chemical and Biological Technologies in Agriculture | / 3卷 / 1期

关键词：

Carrier particles; Enhanced biodegradation; Microbial community; Remediation; Soil; Sulfamethazine;

D O I：

10.1186/s40538-016-0080-6

中图分类号：

学科分类号：

摘要：

Background: The veterinary antibiotic Sulfamethazine (SMZ) contaminates soils via manure applications. Like other soil contaminants (herbicides, fungicides, and nematicides), it has to be degraded. The main challenge is that SMZ biodegradation with bacteria is impeded, since SMZ is a bacteriostatic antibiotic, designed to block microbes in their growth. Results: In this study, we enriched the indigenous soil microbial community (including the single strain Microbacterium sp. C448, adapted to SMZ degradation) from a Canadian soil and we present a suitable approach, for soil remediation by inoculating a German soil with this microbial community established on carrier particles, at environmentally relevant concentrations of 1 mg kg−1. When compared with the isolated SMZ-degrading strain (also obtained from Canada), the microbial community outperformed the mineralization rates of the isolated strain in soil. The negligible soil native SMZ mineralization was successfully increased to 44 and 57 % within 46 days, by the microbial community. The sustainability of this increased SMZ mineralization capacity was proven by the rapid mineralization of a second application of14C-SMZ 112 days after the first. Conclusions: The pronounced SMZ mineralization and the high amount of non-extractable14C-residues (NER) in the inoculated soil indicate that the NER are mainly of biogenic origin (metabolically fixed14C). Therefore, the applied inoculation approach decreased the risk of persistent non-extractable SMZ residues. Together with our former studies, this specific soil inoculation approach was tested for three substances with different physico-chemical properties, indicating that this soil bioremediation technique might also be used for other substances. © The Author(s) 2016.

引用

共 44 条

[1]

Iglesias A., Nebot C., Miranda J., Vazquez B., Cepeda A., Detection and quantitative analysis of 21 veterinary drugs in river water using high-pressure liquid chromatography coupled to tandem mass spectrometry, Environ Sci Pollut Res, 19, 8, pp. 3235-3249, (2012)

[2]

Haller M.Y., Muller S.R., McArdell C.S., Alder A.C., Suter M.J.F., Quantification of veterinary antibiotics (Sulfonamides and trimethoprim) in animal manure by liquid chromatography—mass spectrometry, J Chromatogr A, 952, 1-2, pp. 111-120, (2002)

[3]

Sarmah A.K., Meyer M.T., Boxall A.B.A., A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment, Chemosphere, 65, 5, pp. 725-759, (2006)

[4]

Kummerer K., Antibiotics in the aquatic environment—a review—Part I, Chemosphere, 75, 4, pp. 417-434, (2009)

[5]

Kools S.A.E., Moltmann J.F., Knacker T., Estimating the use of veterinary medicines in the European union, Regul Toxicol Pharmacol, 50, 1, pp. 59-65, (2008)

[6]

German Report on the Consumption of Antimicrobials in Human and Veterinary Medicine, (2012)

[7]

Jorgensen S.E., Halling-Sorensen B., Drugs in the environment, Chemosphere, 40, 7, pp. 691-699, (2000)

[8]

Winckler C., Grafe A., Use of veterinary drugs in intensive animal production, J Soils Sediments, 1, 2, pp. 66-70, (2001)

[9]

Christian T., Schneider R.J., Farber H.A., Skutlarek D., Meyer M.T., Goldbach H.E., Determination of antibiotic residues in manure, soil, and surface waters, Acta Hydrochim Hydrobiol, 31, 1, pp. 36-44, (2003)

[10]

Andersson D.I., Hughes D., Evolution of antibiotic resistance at non-lethal drug concentrations, Drug Resist Update, 15, 3, pp. 162-172, (2012)

← 1 2 3 4 5 →