Sodium butyrate enhances the acidic isoform content of recombinant human erythropoietin produced by Chinese hamster ovary cells

被引：0
作者：
Yingwei Liu
Xiangshan Zhou
Zhiwei Song
Yuanxing Zhang
机构：
[1] East China University of Science and Technology,State Key Laboratory of Bioreactor Engineering
[2] Bioprocessing Technology Institute,undefined
[3] Agency for Science,undefined
[4] Technology and Research,undefined
来源：
Biotechnology Letters | 2014年 / 36卷
关键词：
Acidic isoforms; Butyrate; Chinese hamster ovary cells; Human erythropoietin; Recombinant human erythropoietin; Sialidase; Sodium butyrate;
D O I：
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学科分类号：
摘要：
Sodium butyrate is commonly used in mammalian cell cultures to increase the productivity of recombinant proteins. A Chinese hamster ovary (CHO) cell line producing recombinant human erythropoietin (rhEPO) was cultured in commercial medium. Addition of 0.5 mM butyrate inhibited the over-growth of the cells after the medium was changed from serum-added medium to serum-free medium. At the 6th day, the addition of butyrate lowered the transcriptional level of sialidases I, II, and III compared to that of control groups by 56, 87, and 59 %, respectively. Extracellular sialidase activity was decreased by 53 % by addition of butyrate. The inhibition of cell over-growth and the decrease of extracellular sialidase activity helped to increase the acidic isoform content of rhEPO expressed by this CHO cell strain.
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页码：907 / 911
页数：4
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