Correlation between efficacy and structure of recombinant epitope vaccines against bovine type O foot and mouth disease virus

被引：0

作者：

Mingli Fang

Jianli Li

Hua Wang

Ming Yang

Yongsheng Zhang

Lei Zhou

Hongfei Wei

Guang Yang

Yue Yu

Xuefeng Wei

Yongli Yu

Liying Wang

Min Wan

机构：

[1] Jilin University,Department of Molecular Biology, Norman Bethune College of Medicine

[2] Nanjing Agricultural University,College of Veterinary Medicine

[3] Tianjin Ringpu Bio-technology Co.,National Certified Enterprise Technical Center

[4] Ltd,Department of Immunology, Norman Bethune College of Medicine

[5] Jilin University,undefined

[6] Inner Mongolia Jinyu Group Baoling Bio-pharmaceutical Corporation,undefined

来源：

Biotechnology Letters | 2012年 / 34卷

关键词：

Foot and mouth disease virus; Recombinant epitope vaccine; RGD sequence of B cell epitope; VP1 capsid protein;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

To develop recombinant epitope vaccines against foot-and-mouth disease virus (FMDV), genes coding for six recombinant proteins (rP1–rP6) consisting of different combinations of B cell and T cell epitope from VP1 capsid protein (VP1) of type O FMDV were constructed and the 3D structure of these proteins analyzed. This revealed a surface-exposed RGD sequence of B cell epitopes in all six recombinant proteins as that in VP1 of FMDV and rP1, rP2 and rP4 globally mimicked the backbone conformation of the VP1. rP1, rP2 and rP4 stimulated guinea pigs to produce higher level of neutralizing antibodies capable of protecting suckling mice against FMDV challenge. rP1 stimulated cattle to produce FMDV-neutralizing antibody. The data suggest that an efficient recombinant epitope vaccine against FMDV should share local similarities with the natural VP1 of FMDV.

引用

页码：839 / 847

页数：8

共 58 条

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