Quantitative Determination of Rutin in Biological Samples of Rats Upon Administration of Primula veris L. Herbal Dense Extract

Rutin contents in biological samples of blood plasma, cardiomyocytes, and mitochondria of cardiomyocytes in rats after administration of Primula veris L. dense herbal extract were determined using a quantitative HPLC method. The optimum conditions for quantitative determination of rutin were KH2PO4 buffer (50 mM, pH 4.6), 82:18% (v/v) aqueous to organic phase ratio, 30°C, detection at 205 nm, flow rate 1 mL/min, 12 MPa pressure in chromatographic system, and retention time 6 – 6.5 min. Rutin was found to enter the blood and distribute into the heart, in which its concentration was 5.34 times higher than in blood, and to penetrate mitochondria, in which its concentration was 1.7 times higher than in plasma and 3.18 times lower than in the heart.