A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development

被引：0
作者：
Arfaxad Reyes-Alcaraz
Emilio Y. Lucero Garcia-Rojas
Elizabeth A. Merlinsky
Jae Young Seong
Richard A. Bond
Bradley K. McConnell
机构：
[1] University of Houston,Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy
[2] Korea University,undefined
[3] College of Medicine,undefined
[4] Anam-dong,undefined
[5] Seongbuk-gu,undefined
来源：
Communications Biology | / 5卷
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摘要：
Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.
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