Six maize hybrids and their parental inbred lines, grown under controlled conditions, were tested for chilling tolerance using the chlorophyll fluorescence induction technique. The genotypes were ranked based on the decrease in the Fv/Fm parameter after chilling stress at 5°C. The activities of enzymes playing a role in stress defence mechanisms (catalase, glutathione reductase, ascorbate peroxidase, guaiacol peroxidase and glutathione-S-transferase) were determined in control plants and after 1 day of cold treatment. The results suggest that although there are differences between the genotypes in the activities of almost all the antioxidant enzymes, these differences do not reflect the differences in the chilling tolerance.