Production of Drosophila melanogaster acetylcholinesterase in Saccharomyces cerevisiae

被引：0
|
作者：
K.C.A. Stopps
B.P.G. Curran
S.A. Khalawan
C.A. Malcolm
机构：
[1] University of London,School of Biological Sciences, Queen Mary and Westfield College
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
acetylcholinesterase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Biologically active acetylcholinesterase from Drosophila melanogaster was constitutively expressed from a high copy number plasmid in Saccharomyces cerevisiae. Cellular metabolism and plasmid copy number were unaffected by expression. Based on comparative PMSF inhibition, the Kcat value (6430 mol s−1) was commensurate with human and Torpedo acetylcholinesterases. A 1.7 knt truncated mRNA transcript was observed on Northern blot analysis.
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页码：417 / 422
页数：5
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