Direct electrochemistry and electrocatalysis of hemoglobin on a glassy carbon electrode modified with poly(ethylene glycol diglycidyl ether) and gold nanoparticles on a quaternized cellulose support. A sensor for hydrogen peroxide and nitric oxide

被引：0
作者：
Fangping Li
Mingzhe Nie
Xiulan He
Junjie Fei
Yonglan Ding
Bo Feng
机构：
[1] Xiangtan University,Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry
[2] Xiangtan University,College of Chemical Engineering
来源：
Microchimica Acta | 2014年 / 181卷
关键词：
Gold nanoparticles; Poly ethylene glycol diglycidyl ether; Hemoglobin; Direct electrochemistry; Biosensor;
D O I：
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学科分类号：
摘要：
A glassy carbon electrode was modified with gold nanoparticles (Au-NPs) on a quaternized cellulose support in a film composed of poly(ethylene glycol diglycidyl ether) (PEGDGE), and Hb was immobilized on the Au-NPs. The sensor film was characterized by UV–vis spectra, scanning electron microscopy, and electrochemical impedance spectroscopy. Cyclic voltammetry of the Hb in the Au@Qc/PEGDGE film revealed a pair of well-defined and quasi reversible peaks for the protein heme Fe(III)/Fe(II) redox couple at about −0.333 V (vs. SCE). The sensor film also exhibited good electrocatalytic activity for the reduction of nitric oxide and hydrogen peroxide. The amperometric response of the biosensor depends linearly on the concentration of nitric oxide in the 0.9 to 160 μM range, and the detection limit is as low as 12 nM (at 3σ). The response to hydrogen peroxide is linear in the 59 nM to 4.6 μM concentration range, and the detection limit is 16 nM (at 3σ). This biosensor is sensitive, reproducible, and long-term stable.
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页码：1541 / 1549
页数：8
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