Interference of flavonoids with enzymatic assays for the determination of free fatty acid and triglyceride levels

被引：0
作者：
Elise F. Hoek-van den Hil
Karsten Beekmann
Jaap Keijer
Peter C. H. Hollman
Ivonne M. C. M. Rietjens
Evert M. van Schothorst
机构：
[1] Wageningen University,Division of Toxicology
[2] Wageningen University,Division of Human and Animal Physiology
[3] RIKILT-Institute of Food Safety,undefined
来源：
Analytical and Bioanalytical Chemistry | 2012年 / 402卷
关键词：
Free fatty acids; Triglycerides; Enzymatic assays; Flavonoids; Peroxidase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Flavonoids are bioactive food compounds with potential lipid-lowering effects. Commercially available enzymatic assays are widely used to determine free fatty acid (FFA) and triglyceride (TG) levels both in vivo in plasma or serum and in vitro in cell culture medium or cell lysate. However, we have observed that various flavonoids interfere with peroxidases used in these enzymatic assays, resulting in incorrect lower FFA and TG levels than actually present. Furthermore, addition of isorhamnetin or the major metabolite of the flavonoid quercetin in human and rat plasma, quercetin-3-O-glucuronide, to murine serum also resulted in a significant reduction of the detected TG levels, while a trend was seen for FFA levels. It is concluded that when applying these assays, vigilance is needed and alternative analytical methods, directly assessing FFA or TG levels, should be used for studying the biological effects of flavonoids on FFA and TG levels.
引用
收藏
页码：1389 / 1392
页数：3
相关论文
共 117 条
  • [1] Shmeeda H(2003)Enzymatic assays for quality control and pharmacokinetics of liposome formulations: comparison with nonenzymatic conventional methodologies Methods Enzymol 367 272-292
  • [2] Even-Chen S(2010)Fruit polyphenols and CVD risk: a review of human intervention studies Br J Nutr 104 S28-S39
  • [3] Honen R(2011)Inhibitory effects of flavonoids from Abelmoschus manihot flowers on triglyceride accumulation in 3T3-L1 adipocytes Fitoterapia 82 595-600
  • [4] Cohen R(2010)Ameliorative effects of mulberry ( Biosci Biotechnol Biochem 74 2385-2395
  • [5] Weintraub C(2011) L.) leaves on hyperlipidemia in rats fed a high-fat diet: induction of fatty acid oxidation, inhibition of lipogenesis, and suppression of oxidative stress Mol Nutr Food Res 55 530-540
  • [6] Barenholz Y(2008)Chronic dietary intake of quercetin alleviates hepatic fat accumulation associated with consumption of a western-style diet in C57/BL6J mice Obesity (Silver Spring) 16 2081-2087
  • [7] Chong MF(2009)Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese Zucker rats Diabetologia 52 514-523
  • [8] Macdonald R(2010)Failure of dietary quercetin to alter the temporal progression of insulin resistance among tissues of C57BL/6J mice during the development of diet-induced obesity Eur J Pharm Sci 41 16-22
  • [9] Lovegrove JA(2006)Quercetin enhances adiponectin secretion by a PPAR-gamma independent mechanism Cell Mol Life Sci 63 2847-2858
  • [10] An Y(1996)Chronic quercetin exposure affects fatty acid catabolism in rat lung Chem Res Toxicol 9 16-23
12345678910