Thirty-four Galactic star-forming regions have been observed in the J=4−3 line of cyanoacetylene (HC3N) at 36.4 GHz. Emission was detected toward 17 sources. The HC3N column density was determined in the detected sources, and in eight of them (NGC 2264, L379, W51E1/E2, DR 21 West, DR 21 (OH), DR 21, S140, and Cep A), the relative cyanoacetylene abundance was estimated. The HC3N abundance in these objects is about 1–70×10−10.