Labeling of oligonucleotides with fluorescein

被引：0
|
作者：
Patricia M. E. Chadwick
Ian Durrant
机构：
[1] Amersham International plc,Life Sciences Research and Development
来源：
Molecular Biotechnology | 1997年 / 7卷
关键词：
Probe labeling; fluorescein; hybridization; oligonucleotide;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The labeling of oligonucleotide probes using a fluorescein-labeled nucleotide is described. The reaction is characterized by careful control of the nucleotide and probe molar ratio in order to produce a tail that gives good detection sensitivity without compromising hybridization stringency control of the probe sequence. The labeling reaction can be easily monitored for incorporation of the fluorescent label and the probes can be used in many applications.
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页码：299 / 301
页数：2
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