Preliminary Pollen Analysis of Some Apple Cultivars in Kashmir: Towards Understanding the Apple Pollen Morphology

被引：2
作者：
Dar J.A. [1 ]
Wani A.A. [1 ]
Dhar M.K. [2 ]
机构：
[1] Cytogenetics and Reproductive Biology Laboratory, Department of Botany, University of Kashmir, Hazratbal, Srinagar
[2] School of Biotechnology, University of Jammu, Jammu
来源：
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2020年 / 90卷 / 2期
关键词：
Cultivar identification; Malus; ×; domestica; Pollen morphology; SEM;
D O I：
10.1007/s40011-019-01117-6
中图分类号：
学科分类号：
摘要：
Kashmir Valley, a North Western state in India, represents the richest repository of apple germplasm of about 200 cultivars besides some unknown genotypes of seedling origin. Among the rich germplasm resource, sixteen apple cultivars belonging to two commercially important groups with one group having Delicious as a suffix and other group having Ambri as a suffix were examined for the first time based on pollen grain surface morphology using scanning electron microscopy for genotype differentiation. Most of the air-dried apple pollen grains were elliptical and tricolpate with three germinal furrows extending almost the full length of the pollen grain. However, round and tricolpate pollen grains were observed in cultivars Kullu Delicious, Oregon Spur, Reeka Red, and Silver Spur. Striation pattern of the pollen grains varies from cultivar to cultivar. Mostly, the striations extend from one end of colpi to another end, but in case of Cross Delicious, the striations were irregular and form network-like structures. Pores on the exine surface also showed great variations among the cultivars. The cultivars like Oregon Spur, Silver Spur, and Reeka Red are said to be the sports of Delicious, but from the results of scanning electron microscopy, these resemble Kullu Delicious in terms of pollen shape, striation pattern and pores on the exine surface. © 2019, The National Academy of Sciences, India.
引用
收藏
页码：431 / 438
页数：7
相关论文
共 22 条
  • [1] Way R.D., Aldwinckle H.S., Lamb R.C., Rejman A., Sansavini S., Shen T., Apples (Malus), Genetic resources of temperate fruit and nut, pp. 1-62, (1990)
  • [2] Janick J., Cummins J.N., Brown S.K., Hemmat M., Fruit breeding, Fruit breeding: tree and tropical fruits, pp. 1-77, (1996)
  • [3] Evrenosoglu Y., Misirli A., Investigations on the pollen morphology of some fruit species, Turk J Agric For, 33, pp. 181-190, (2009)
  • [4] Jahangir A.D., Aijaz A.W., Manoj K.D., Morphological, biochemical and male-meiotic characterization of apple (Malus × domestica Borkh.) germplasm of Kashmir Valley, Chromosome Bot, 10, pp. 39-49, (2015)
  • [5] Jahangir A.D., Aijaz A.W., Manoj K.D., Male meiotic behaviour of apple germplasm in Kashmir: towards understanding the evolution of apple, Cytologia, 83, 1, pp. 87-91, (2018)
  • [6] Fogle H.W., Identification of clones within four tree fruit species by pollen exine patterns, J Am Soc Hortic Sci, 102, 5, pp. 552-560, (1977)
  • [7] Perveen A., Qaiser M., Pollen flora of Pakistan—XVII. Illecebraceae, Pak J Bot, 35, pp. 141-144, (2003)
  • [8] Martens J., Fretz T.A., Identification of eight crabapples by pollen surface sculpture, J Am Soc Hortic Sci, 105, pp. 257-263, (1980)
  • [9] Lanza B., Marsilio V., Martinolli N., Olive pollen ultrastructure: characterization of exine pattern through image analysis-scanning electron microscope (IA-SEM), Sci Hortic, 65, pp. 283-294, (1996)
  • [10] Javady T., Arzani K., Pollen morphology of five Iranian olive (Olea europaea L.) cultivars, J Agric Sci Technol, 3, pp. 37-42, (2001)
123