Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization

被引：0
|
作者：
Yoshihiro Kaku
Akira Noguchi
Glenn A. Marsh
Jennifer A. Barr
Akiko Okutani
Kozue Hotta
Boldbaatar Bazartseren
Christopher C. Broder
Akio Yamada
Satoshi Inoue
Lin-Fa Wang
机构：
[1] National Institute of Infectious Diseases,Department of Veterinary Science
[2] CSIRO Livestock Industries,Australian Animal Health Laboratory
[3] Uniformed Services University,Department of Microbiology and Immunology
来源：
Archives of Virology | 2012年 / 157卷
关键词：
Vesicular Stomatitis Virus; Envelope Glycoprotein; Live Virus; ELISA System; Nipah Virus;
D O I：
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中图分类号：
学科分类号：
摘要：
A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2.5 × 104 TCID50. Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.
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页码：1605 / 1609
页数：4
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