Development in a Primary Cell Culture of the Marine Sponge Ircinia muscarum and Analysis of the Polar Compounds

被引：0

作者：

Salvatore De Rosa

Salvatore De Caro

Giuseppina Tommonaro

Krasimir Slantchev

Kamen Stefanov

Simeon Popov

机构：

[1] Istituto per la Chimica di Molecole di Interesse Biologico,

[2] CNR,undefined

[3] via Toiano 6,undefined

[4] 80072 Arco Felice,undefined

[5] Napoli,undefined

[6] Italy,undefined

[7] Institute of Organic Chemistry with Centre of Phytochemistry,undefined

[8] Bulgarian Academy of Sciences,undefined

[9] Sofia 1113,undefined

[10] Bulgaria,undefined

来源：

Marine Biotechnology | 2001年 / 3卷

关键词：

Key words: sponge cell cultures, growth promotion, Ircinia muscarum, polar compounds, biotechnology.;

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学科分类号：

摘要：

We have established a primary cell culture of the marine demosponge Ircinia muscarum. The culture was started from a cell suspension obtained by a combination of mechanical chemical means. Microbial contamination was controlled by the use of a pool of antibiotics. Optical density, rather than hemocytometer count, is suggested to monitor the cellular growth. Analysis of the chemical composition of I. muscarum cells revealed absence of sterols, showing that the cells were unable to biosynthesize sterols. When the medium was supplemented with cholesterol an increase of about 70% in the number of cells was observed. These results suggest that the classic mammalian nutrient medium was not satisfactory for I. muscarum cell growth, and sterols were needed to satisfy the membrane requirements.

引用

页码：281 / 286

页数：5