Decreased deformability of lymphocytes in chronic lymphocytic leukemia

被引：0
作者：
Yi Zheng
Jun Wen
John Nguyen
Mark A. Cachia
Chen Wang
Yu Sun
机构：
[1] University of Toronto,Department of Mechanical and Industrial Engineering
[2] Institute of Biomaterials and Biomedical Engineering,Department of Electrical and Computer Engineering
[3] University of Toronto,Department of Pathology and Laboratory Medicine
[4] University of Toronto,Department of Laboratory Medicine and Pathobiology
[5] Mount Sinai Hospital,undefined
[6] University of Toronto,undefined
来源：
Scientific Reports | / 5卷
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摘要：
This paper reports the first study of stiffness/deformability changes of lymphocytes in chronic lymphocytic leukemia (CLL) patients, demonstrating that at the single cell level, leukemic metastasis progresses are accompanied by biophysical property alterations. A microfluidic device was utilized to electrically measure cell volume and transit time of single lymphocytes from healthy and CLL patients. The results from testing thousands of cells reveal that lymphocytes from CLL patients have higher stiffness (i.e., lower deformability), as compared to lymphocytes in healthy samples, which was also confirmed by AFM indentation tests. This observation is in sharp contrast to the known knowledge on other types of metastatic cells (e.g., breast and lung cancer cells) whose stiffness becomes lower as metastasis progresses.
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