Mouse A6-positive hepatic oval cells derived from embryonic stem cells

被引:0
|
作者
Dong-zhi Yin
Ji-ye Cai
Qi-chang Zheng
Zheng-wei Chen
Jing-xian Zhao
You-neng Yuan
机构
[1] Huangshi Central Hospital,Department of General Surgery
[2] Huazhong University of Science and Technology,Department of General Surgery, Union Hospital, Tongji Medical College
[3] Jinan University,College of Life Science and Technology
[4] University of Illinois,Department of Microbiology and Immunology
[5] College of Medicine,undefined
来源
Journal of Huazhong University of Science and Technology [Medical Sciences] | 2014年 / 34卷
关键词
hepatic oval cells; embryonic stem cells; cell differentiation;
D O I
暂无
中图分类号
学科分类号
摘要
Oval cells have a potential to differentiate into a variety of cell lineages including hepatocytes and biliary epithelia. Several models have been established to activate the oval cells by incorporating a variety of toxins and carcinogens, alone or combined with surgical treatment. Those models are obviously not suitable for the study on human hepatic oval cells. It is necessary to establish a new and efficient model to study the human hepatic oval cells. In this study, the hepatocyte growth factor (HGF) and epidermal growth factor (EGF) were used to induce differentiation of mouse embryonic stem (ES) cells into hepatic oval cells. We first confirmed that hepatic oval cells derived from ES cells, which are bipotential, do exist during the course of mouse ES cells’ differentiation into hepatic parenchymal cells. RT-PCR and transmission electron microscopy were applied in this study. The ratio of Sca-1+/CD34+ cells sorted by FACS in the induction group was increased from day 4 and reached the maximum on the day 8, whereas that in the control group remained at a low level. The differentiation ratio of Sca-1+/CD34+ cells in the induction group was significantly higher than that in the control group. About 92.48% of the sorted Sca-1+/CD34+ cells on the day 8 were A6 positive. Highly purified A6+/Sca-1+/CD34+ hepatic oval cells derived from ES cells could be obtained by FACS. The differentiation ratio of hepatic oval cells in the induction group (up to 4.46%) was significantly higher than that in the control group. The number of hepatic oval cells could be increased significantly by HGF and EGF. The study also examined the ultrastructures of ES-derived hepatic oval cells’ membrane surface by atomic force microscopy. The ES-derived hepatic oval cells cultured and sorted by our protocols may be available for the future clinical application.
引用
收藏
页码:1 / 9
页数:8
相关论文
共 50 条
  • [41] An experimental study on astrocytes promoting production of neural stem cells derived from mouse embryonic stem cells
    ZHOU Yufeng FANG Feng FU Jinrong DONG Yongsui YE Duyun SHU Sainan ZHEN Hong and LI GeDepartment of Pediatrics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Pathophysiology Tongji Medical College Huazhong University of Science and Technology Wuhan China
    Chinese Medical Journal, 2005, (23) : 1994 - 1999
  • [42] An experimental study on astrocytes promoting production of neural stem cells derived from mouse embryonic stem cells
    Zhou, YF
    Fang, F
    Fu, JR
    Dong, YS
    Ye, DY
    Shu, SN
    Zhen, H
    Li, G
    CHINESE MEDICAL JOURNAL, 2005, 118 (23) : 1994 - 1999
  • [43] Factors derived from mouse embryonic stem cells promote self-renewal of goat embryonic stem-like cells
    Tian, Hai-Bin
    Wang, Hong
    Sha, Hong-Ying
    Xu, Xu-Jun
    Zhu, Min
    Wu, You-Bing
    Cheng, Sheng-Hua
    Chen, Jian-Quan
    Shi, Yong-Xiang
    Bai, Zeng-Liang
    Cheng, Guo-Xiang
    CELL BIOLOGY INTERNATIONAL, 2006, 30 (05) : 452 - 458
  • [44] In vitro differentiation of hepatic progenitor cells from mouse embryonic stem cells induced by sodium butyrate
    Zhou, Qing-Jun
    Xiang, Li-Xin
    Shao, Jian-Zhong
    Hu, Ruo-Zhen
    Lu, Yong-Liang
    Yao, Hang
    Dai, Li-Cheng
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2007, 100 (01) : 29 - 42
  • [45] Effects of Sodium Butyrate on the Differentiation of Pancreatic and Hepatic Progenitor Cells From Mouse Embryonic Stem Cells
    Ren, Meng
    Yan, Li
    Shang, Chang-Zhen
    Cao, Jun
    Lu, Li-Hong
    Min, Jun
    Cheng, Hua
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2010, 109 (01) : 236 - 244
  • [46] Transplantation of Flk1-Positive Mesodermal Cells Derived from Mouse Embryonic Stem Cells Improves Diabetic Polyneuropathy in Mice
    Himeno, Tatsuhito
    Kamiya, Hideki
    Suzuki, Hirohiko
    Kito, Tetsutaro
    Naruse, Keiko
    Shibata, Taiga
    Kondo, Masaki
    Kato, Jiro
    Okawa, Tetsuji
    Isobe, Kenichi
    Oiso, Yutaka
    Nakamura, Jiro
    DIABETES, 2010, 59 : A263 - A263
  • [47] The genomic and proteomic blueprint of mouse megakaryocytes derived from embryonic stem cells
    Merico, V.
    Zuccotti, M.
    Carpi, D.
    Baev, D.
    Mulas, F.
    Sacchi, L.
    Bellazzi, R.
    Pastorelli, R.
    Redi, C. A.
    Moratti, R.
    Garagna, S.
    Balduini, A.
    JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2012, 10 (05) : 907 - 915
  • [48] Mesoderm derived from mouse embryonic stem cells displays nephrogenic potential
    Rak-Raszewska, Aleksandra
    Murray, Patricia
    MECHANISMS OF DEVELOPMENT, 2009, 126 : S239 - S239
  • [49] Gabaergic neurons derived from mouse embryonic stem cells: A brief characterization
    Haynes, John
    Khaira, Simer
    Tochon-Danguy, Nathalie
    Pouton, Colin
    ACTA PHARMACOLOGICA SINICA, 2006, 27 : 238 - 238
  • [50] MOLECULAR ANALYSIS OF KERATINOCYTES WHICH DERIVED FROM MOUSE EMBRYONIC STEM CELLS
    Vatansever, H. Seda
    Turkoz-Uluer, Elgin
    Aydede, Hasan
    Ozbilgin, M. Kemal
    IUBMB LIFE, 2009, 61 (03) : 338 - 338