Proteomic analysis of cisplatin resistance in human ovarian cancer using 2-DE method

被引：0
作者：
Fengming Gong
Xingchen Peng
Zhi Zeng
Ming Yu
Yuwei Zhao
Aiping Tong
机构：
[1] Sichuan University,State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School
[2] Guizhou Provincial People’s Hospital,Department of Oncology
[3] Sichuan University,Department of Hematology, West China Hospital, West China Medical School
来源：
Molecular and Cellular Biochemistry | 2011年 / 348卷
关键词：
Cisplatin; Drug resistance; Human ovarian cancer; Two-dimensional gel electrophoresis; Proteomics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Platinum-based chemotherapy, such as cisplatin, is the primary treatment for human ovarian cancer. However, overcoming drug resistance has become an important issue in cancer chemotherapy. In this study, we performed 2-DE and ESI-Q-TOF MS/MS analysis to identify differential proteins expression between cisplatin-sensitive (A2780S) and cisplatin-resistant (A2780-CP) ovarian cancer cell lines. Of the 14 spots identified as differentially expressed (±over twofold, P < 0.05) between the two cell lines, ten spots (corresponding to ten unique proteins) were positively identified by ESI-Q-TOF MS/MS analysis. These proteins include capsid glycoprotein, fructose-bisphosphate aldolase C, heterogeneous nuclear ribonucleoproteins A2/B1, putative RNA-binding protein 3, Ran-specific GTPase-activating protein, ubiquitin carboxyl-terminal hydrolase isozyme L1, stathmin, ATPSH protein, chromobox protein homolog3 and phosphoglycerate kinase 1. The proteins identified in this study would be useful in revealing the mechanisms underlying cisplatin resistance and also provide some clues for further research.
引用
收藏
页码：141 / 147
页数：6
相关论文
共 187 条
[11]  
Skinner MK(2005)Perturbation of the tumor necrosis factor-related apoptosis-inducing ligand cascade in ovarian cancer: overexpression of FLIPL and deregulation of the functional receptors DR4 and DR5 Mol Clin Cancer Res 11 8585-8591
[12]  
Henkels KM(2004)Applying proteomics to signaling networks Curr Opin Genet Dev 14 492-498
[13]  
Turchi JJ(1997)Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients Electrophoresis 18 307-316
[14]  
Agarwal R(1987)Elimination of point straking on silver-stained two-dimensional gels by addition of iodoacetamide to the equilibration buffer Electrophoresis 8 122-124
[15]  
Kaye SB(2008)Proteomic analysis of cellular protein alterations using a hepatitis B virus-producing cellular model Proteomics 8 2012-2023
[16]  
Hajra KM(2008)Proteomic analysis of liver cancer cells treated with suberonylanilide hydroxamic acid Cancer Chemother Pharmacol 61 791-802
[17]  
Tan L(2003)Role of BRCA1 in cellular resistance to paclitaxel and ionizing radiation in an ovarian cancer cell line carrying a defective BRCA1 Oncogene 22 2396-2404
[18]  
Liu JR(2002)A targeted inhibition of DNA-dependent protein kinase sensitizes breast cancer cells following ionizing radiation J Pharmacol Exp Ther 303 753-759
[19]  
Hall MD(2003)Disruption of the Fanconi anemia-BRCA pathway in cisplatin-sensitive ovarian tumors Nat Med 9 568-574
[20]  
Mellor HR(2006)Heterologous expression of a mammalian ABC transporter in plant and its application to phytoremediation Plant Mol Biol 61 491-503
12345678910