Development of genome-wide SNPs for population genetics and population assignment of Sebastiscus marmoratus

被引：0
作者：
Shanshan Cai
Shengyong Xu
Lu Liu
Tianxiang Gao
Yongdong Zhou
机构：
[1] Zhejiang Ocean University,Fishery College
[2] Ocean University of China,Institute of Evolution &Marine Biodiversity
[3] Ministry of Agriculture,Key Laboratory for Technology Research on Sustainable Utilization of Marine Fishery Resources, Marine Fisheries Research Institute of Zhejiang, Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Groun
来源：
Conservation Genetics Resources | 2018年 / 10卷
关键词：
Marbled rockfish; Next-generation sequencing; Single nucleotide polymorphism; Population assignment;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The assessment of genetic structure and genetic diversity is crucial for management and conservation. A low number of markers may fail to identify robust population structure. A solution of this shortcoming can be high-throughput sequencing that allows genotyping thousands of markers on a genome-wide approach. We used Genotyping-by-sequencing (GBS) approach on 60 individuals of Sebastiscus marmoratus, from three locations in Chinese coastal waters. We discovered a total of 7376 genome-wide single nucleotide polymorphisms (SNPs). After applying careful filtering criteria, we obtained 298 high-differentiated SNPs that performed well for population genetics and population assignment. The 298 SNPs provided high power for population assignment with only one misassignment among three populations. SNP validation confirmed the existence and the high quality of this filtered SNPs. The SNPs in our study could be used as powerful markers in further genetic and evolutionary studies.
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页码：575 / 578
页数：3
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