In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant

被引：3
作者：
Sompop Prathanturarug
Rungthipwan Pheakkoet
Thaya Jenjittikul
Wongsatit Chuakul
Promchit Saralamp
机构：
[1] Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok 10400
[2] Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400
来源：
Physiology and Molecular Biology of Plants | 2012年 / 18卷 / 3期
关键词：
Conservation; Medicinal plant; Stemona hutanguriana; Stemonaceae; Tissue culture;
D O I：
10.1007/s12298-012-0116-8
中图分类号：
学科分类号：
摘要：
In vitro propagation of Stemona hutanguriana W. Chuakul, an endangered species of Stemonaceae, was established. Nodal and internodal explants were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) alone or in combination with 1-naphthaleneacetic acid (NAA). When cultured on MS medium supplemented with 18. 16 μM TDZ with 0. 54 μM NAA for 8 weeks and then on MS medium without plant growth regulator (PGR) for 8 weeks, nodal explants demonstrated a responding frequency of 91. 67 % and a shoot regeneration rate of 5. 46 shoots/responding explant. Furthermore, internodal explants demonstrated a responding frequency of 17. 17 % with 11. 17 shoots/responding explant. The regenerated shoots were rooted using a two-step protocol by culturing for 4 weeks on MS medium supplemented with various concentrations of NAA and Indole-3-butyric acid (IBA) and 8 weeks on MS medium without PGR. When 5. 40 μM NAA was used as a PGR, the maximum root induction rate was 69. 45 %. Regenerated plantlets were successfully transferred to soil. © 2012 Prof. H.S. Srivastava Foundation for Science and Society.
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页码：281 / 286
页数：5
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