Development and characterisation of EST-SSR markers by transcriptome sequencing in taro (Colocasia esculenta (L.) Schoot)

被引：0
作者：
Yongning You
Duchen Liu
Huabo Liu
Xingfei Zheng
Ying Diao
Xinfang Huang
Zhongli Hu
机构：
[1] Wuhan University,State Key Laboratory of Hybrid Rice, College of Life Sciences
[2] Sichuan Academy of Agricultural Sciences,Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province, Horticulture Institute
[3] Hubei Engineering University,Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, College of Agricultural
[4] Wuhan Vegetable Institute of Science,Wuhan National Germplasm Repository for Aquatic Vegetables
来源：
Molecular Breeding | 2015年 / 35卷
关键词：
Taro; Simple sequence repeat marker; Transcriptome; Genetic diversity;
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摘要：
Taro (Colocasia esculenta) is an important crop with a long history of cultivation. In this study 5278 SSRs were identified in taro transcriptome data. A total of 2858 primer pairs were designed for marker development. 100 primers were randomly selected and synthesized. Among them, 72 primer pairs were successfully amplified and 62 were polymorphic in taro accessions. The number of alleles ranged from 2 to 14 for each different polymorphic locus and the polymorphism information content valued ranged from 0.01 to 0.82. The phylogenetic tree was also constructed to analyse the genetic diversity in 68 taro accessions. The large number of taro SSR markers developed in the present study will be useful in the researches of genetic diversity, germplasm characterization and molecular breeding etc.
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[1]  
Aggarwal RK(2007)Identification, characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species Theor Appl Genet 114 359-372
[2]  
Hendre PS(2013)Identification of chloroplast genome loci suitable for high-resolution phylogeographic studies of Mol Ecol Resour 13 929-937
[3]  
Varshney RK(1980) (L.) Schott (Araceae) and closely related taxa Am J Hum Genet 32 314-854
[4]  
Bhat PR(2000)Construction of a genetic linkage map in man using restriction fragment length polymorphisms Genetics 156 847-132
[5]  
Krishnakumar V(2011)Computational and experimental characterization of physically clustered simple sequence repeats in plants BMC Plant Biol 99 125-43
[6]  
Singh L(2007)Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [ Heredity 119 39-97
[7]  
Ahmed I(2001) (L.) Millspaugh] Euphytica 23 85-749
[8]  
Matthews PJ(2009)EST-SSRs as a resource for population genetic analyses Mol Breed 109 740-1358
[9]  
Biggs PJ(2004)Assessment of genotypic variation among cultivated durum wheat based on EST-SSRS and genomic SSRS Theor Appl Genet 29 1355-584
[10]  
Naeem M(2008)Development, characterization and cross-species/genera transferability of EST-SSR markers for rubber tree ( Electrophoresis 9 582-1707
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