Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig

被引：0
作者：
Kateřina Svobodová
Karel Bílek
Aleš Knoll
机构：
[1] Mendel University of Agriculture and Forestry,Department of Animal Morphology, Physiology and Genetics
来源：
Journal of Applied Genetics | 2008年 / 49卷
关键词：
gene stability; real-time PCR; reference genes;
D O I：
暂无
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学科分类号：
摘要：
The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes:EEF1A1, GAPDH, HPRT1 andTOP2B. The level of expression was characterized byCt values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.
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页码：263 / 265
页数：2
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