Engineering a predatory bacterium as a proficient killer agent for intracellular bio-products recovery: The case of the polyhydroxyalkanoates

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作者：
Virginia Martínez
Cristina Herencias
Edouard Jurkevitch
M. Auxiliadora Prieto
机构：
[1] Centro de Investigaciones Biológicas,Environmental Biology Department
[2] Faculty of Agricultural,Department of Plant Pathology and Microbiology
[3] Food and Environmental Quality Sciences,undefined
[4] the Hebrew University of Jerusalem,undefined
[5] Present address: V. Martínez,undefined
[6] Novo Nordisk Foundation Center for Biosustainability,undefined
[7] Technical University of Denmark,undefined
[8] Hørsholm,undefined
[9] Denmark.,undefined
来源：
Scientific Reports | / 6卷
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摘要：
This work examines the potential of the predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator of other Gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) produced naturally by Pseudomonas putida and Cupriavidus necator, or by recombinant Escherichia coli strains. B. bacteriovorus with a mutated PHA depolymerase gene to prevent the unwanted breakdown of the bio-product allowed the recovery of up to 80% of that accumulated by the prey bacteria, even at high biomass concentrations. This innovative downstream process highlights how B. bacteriovorus can be used as a novel, biological lytic agent for the inexpensive, industrial scale recovery of intracellular products from different Gram-negative prey cultures.
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