Role of tumor metastasis suppressor gene KAI1 in digestive tract carcinomas and cancer cells

被引:0
作者
Qiao Wu
Yuan Ji
Ming-qing Zhang
Yu-qiang Chen
Fu Chen
Da-lin Shi
Zong-hui Zheng
Yao-jian Huang
Wen-jin Su
机构
[1] Xiamen University,Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences
[2] Dong Nan Hospital,undefined
[3] 174 Hospital,undefined
来源
Cell and Tissue Research | 2003年 / 314卷
关键词
KAI1; p53; VP-16; Digestive tract carcinoma; Human;
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摘要
The KAI1 gene is identified as a tumor metastasis suppressor gene in many types of cancer. We examined KAI1 gene and its protein KAI1/CD82 expression by in situ hybridization and immunohistochemical analysis, and found that KAI1 mRNA and protein expression were inversely correlated with lymph node and distant metastasis in digestive tract carcinomas, but not with age and gender of the patient, or with tumor differentiation. Moreover, KAI1/CD82 protein expression positively reflected the survival outcome of patients. Western blot analysis showed that VP-16 increased KAI1/CD82 protein expression obviously in various cancer cell lines, especially in those that were highly metastatic. This increased KAI1/CD82 expression was associated with its translocation from the cytomembrane to the nucleus, in which it interacted with nuclear p53 protein, forming a strong complex, observed by confocal microscopy and co-immunoprecipitation, respectively. In nude mice, after feeding with VP-16, the number of tumors metastasized from spleen to liver was obviously reduced, and KAI1/CD82 protein expression became stronger in those metastatic tumors. Accordingly, this demonstrated that KAI1 might be used as an indicator for predicting the clinical outcome, and VP-16 may be clinically considered as a promising candidate for anti-metastasis with regard to its potential to upregulate KAI1 expression.
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页码:237 / 249
页数:12
相关论文
共 56 条
[1]  
Adachi XH(1996)Functional p53 is required for triptolide-induced apoptosis and AP-1 and nuclear factor-kappaB activation in gastric cancer cells Cancer Res 56 1751-8018
[2]  
Arrowsmith BC(1996)Nuclear factor-kappaB-dependent expression of metastasis suppressor KAI1/CD82 gene in lung cancer cell lines expressing mutant p53 Oncogene 12 1379-678
[3]  
Aznavoorian MC(1993)undefined Cancer 71 1368-undefined
[4]  
Carter GH(1990)undefined J Urol 143 742-undefined
[5]  
Chen HF(2000)undefined Chin Med J 345-undefined
[6]  
Chresta SH(1996)undefined Cancer Res 56 1834-undefined
[7]  
Dong D(1995)undefined Science 268 884-undefined
[8]  
Dong SK(1996)undefined Cancer Res 56 4387-undefined
[9]  
Dong T(1997)undefined Genomics 41 25-undefined
[10]  
Engel T(1994)undefined Leuk Lymphoma 13 Suppl 1 61-undefined