Wavelength-Resolved 3-Dimensional Fluorescence Lifetime Imaging

被引:0
|
作者
S. E. D. Webb
S. Lévêque-Fort
D. S. Elson
J. Siegel
T. Watson
M. J. Lever
M. Booth
R. Juskaitis
M. A. A. Neil
L. O. Sucharov
T. Wilson
P. M. W. French
机构
[1] Imperial College of Science,Photonics Group, Department of Physics
[2] Technology and Medicine,Department of Conservative Dentistry, Guys, Kings and St. Thomas'
[3] Dental Institute,Department of Bioengineering
[4] Imperial College of Science,Department of Engineering Science
[5] Technology and Medicine,undefined
[6] University of Oxford,undefined
来源
Journal of Fluorescence | 2002年 / 12卷
关键词
Fluorescence lifetime imaging; wavelength-resolved imaging; 3-dimensional imaging, teeth, caries;
D O I
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中图分类号
学科分类号
摘要
We have developed a compact system for wide-field fluorescence imaging, resolved in three spatial dimensions, lifetime and wavelength, that is based on a gated optical intensifier and an all-solid-state diode pumped Cr:LiSAF oscillator-amplifier system. Exploiting spectral separation, the system has been applied to human teeth, obtaining good lifetime contrast between enamel, dentin and caries. Exploiting spectral separation combined with depth resolution, the study of fluorescent microspheres led to an enhancement in both lifetime contrast and lateral resolution.
引用
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页码:279 / 283
页数:4
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