Wavelength-Resolved 3-Dimensional Fluorescence Lifetime Imaging

被引：0
|
作者：
S. E. D. Webb
S. Lévêque-Fort
D. S. Elson
J. Siegel
T. Watson
M. J. Lever
M. Booth
R. Juskaitis
M. A. A. Neil
L. O. Sucharov
T. Wilson
P. M. W. French
机构：
[1] Imperial College of Science,Photonics Group, Department of Physics
[2] Technology and Medicine,Department of Conservative Dentistry, Guys, Kings and St. Thomas'
[3] Dental Institute,Department of Bioengineering
[4] Imperial College of Science,Department of Engineering Science
[5] Technology and Medicine,undefined
[6] University of Oxford,undefined
来源：
Journal of Fluorescence | 2002年 / 12卷
关键词：
Fluorescence lifetime imaging; wavelength-resolved imaging; 3-dimensional imaging, teeth, caries;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have developed a compact system for wide-field fluorescence imaging, resolved in three spatial dimensions, lifetime and wavelength, that is based on a gated optical intensifier and an all-solid-state diode pumped Cr:LiSAF oscillator-amplifier system. Exploiting spectral separation, the system has been applied to human teeth, obtaining good lifetime contrast between enamel, dentin and caries. Exploiting spectral separation combined with depth resolution, the study of fluorescent microspheres led to an enhancement in both lifetime contrast and lateral resolution.
引用
收藏
页码：279 / 283
页数：4
相关论文
共 50 条
  • [1] Wavelength-resolved 3-dimensional fluorescence lifetime imaging
    Webb, SED
    Lévêque-Fort, S
    Elson, DS
    Siegel, J
    Watson, T
    Lever, MJ
    Booth, M
    Juskaitis, R
    Neil, MAA
    Sucharov, LO
    Wilson, T
    French, PMW
    JOURNAL OF FLUORESCENCE, 2002, 12 (02) : 279 - 283
  • [2] Wavelength-resolved measurements of fluorescence lifetime of indocyanine green
    Gerega, Anna
    Zolek, Norbert
    Soltysinski, Tomasz
    Milej, Daniel
    Sawosz, Piotr
    Toczylowska, Beata
    Liebert, Adam
    JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (06)
  • [3] WAVELENGTH-RESOLVED FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS
    TIMPERMAN, AT
    KHATIB, K
    SWEEDLER, JV
    ANALYTICAL CHEMISTRY, 1995, 67 (01) : 139 - 144
  • [4] Capillary electrophoresis with wavelength-resolved fluorescence detection
    Timperman, AT
    Sweedler, JV
    ANALYST, 1996, 121 (05) : R45 - R52
  • [5] Characterizing submicron vesicles with wavelength-resolved fluorescence in flow cytometry
    Fuller, RR
    Sweedler, JV
    CYTOMETRY, 1996, 25 (02): : 144 - 155
  • [6] Wavelength-Resolved PhotoATRP
    Nardi, Martina
    Blasco, Eva
    Barner-Kowollik, Christopher
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2022, 144 (03) : 1094 - 1098
  • [7] Wavelength-resolved fluorescence detector for microchip capillary electrophoresis separations
    Gotz, Sebastian
    Karst, Uwe
    SENSORS AND ACTUATORS B-CHEMICAL, 2007, 123 (01) : 622 - 627
  • [8] Advanced Corrections of wavelength-resolved neutron transmission imaging
    Ala’a Al-Falahat
    Nikolay Kardjilov
    Robin Woracek
    Saad Alrwashdeh
    Talib K. Murtadha
    Ingo Manke
    The European Physical Journal Plus, 138
  • [9] Metabolic Profiling of the Skin to Monitor the Onset and Progression of Squamous Cell Carcinoma through Time- and Wavelength-Resolved Fluorescence Lifetime Imaging
    Miller, Christina R.
    Nichols, Michael G.
    BIOPHYSICAL JOURNAL, 2015, 108 (02) : 478A - 478A
  • [10] Advanced Corrections of wavelength-resolved neutron transmission imaging
    Al-Falahat, Ala'a
    Kardjilov, Nikolay
    Woracek, Robin
    Alrwashdeh, Saad
    Murtadha, Talib K.
    Manke, Ingo
    EUROPEAN PHYSICAL JOURNAL PLUS, 2023, 138 (09):
12345