Simple sequence repeat markers that identify claviceps species and strains

被引：10

作者：

Gilmore B.S. ^{[1
]}

Alderman S.C. ^{[1
]}

Knaus B.J. ^{[2
]}

Bassil N.V. ^{[3
]}

Martin R.C. ^{[1
]}

Dombrowski J.E. ^{[1
]}

Dung J.K.S. ^{[4
]}

机构：

[1] USDA ARS Forage Seed and Cereal Research, 3450 SW Campus Way, Corvallis, 97331, OR

[2] USDA ARS Horticultural Crops Research Unit, 3420 NW Orchard Ave, Corvallis, 97331, OR

[3] USDA ARS National Clonal Germplasm Repository, 33447 Peoria Road, Corvallis, 97333, OR

[4] Oregon State University, Central Oregon Agricultural Research Center, 850 NW Dogwood Lane, Madras, 97741, OR

来源：

Fungal Biology and Biotechnology | / 3卷 / 1期

关键词：

Claviceps humidiphila; Claviceps purpurea; ERGOT; Kentucky bluegrass; Perennial ryegrass; Simple sequence repeat primers;

D O I：

10.1186/s40694-016-0019-5

中图分类号：

学科分类号：

摘要：

Background: Claviceps purpurea is a pathogen that infects most members of Pooideae, a subfamily of Poaceae, and causes ergot, a floral disease in which the ovary is replaced with a sclerotium. When the ergot body is accidently consumed by either man or animal in high enough quantities, there is extreme pain, limb loss and sometimes death. Results: This study was initiated to develop simple sequence repeat (SSRs) markers for rapid identification of C. purpurea. SSRs were designed from sequence data stored at the National Center for Biotechnology Information database. The study consisted of 74 ergot isolates, from four different host species, Lolium perenne, Poa pratensis, Bromus inermis, and Secale cereale plus three additional Claviceps species, C. pusilla, C. paspali and C. fusiformis. Samples were collected from six different counties in Oregon and Washington over a 5-year period. Thirty-four SSR markers were selected, which enabled the differentiation of each isolate from one another based solely on their molecular fingerprints. Discriminant analysis of principle components was used to identify four isolate groups, CA Group 1, 2, 3, and 4, for subsequent cluster and molecular variance analyses. CA Group 1 consisting of eight isolates from the host species P. pratensis, was separated on the cluster analysis plot from the remaining three groups and this group was later identified as C. humidiphila. The other three groups were distinct from one another, but closely related. These three groups contained samples from all four of the host species. These SSRs are simple to use, reliable and allowed clear differentiation of C. humidiphila from C. purpurea. Isolates from the three separate species, C. pusilla, C. paspali and C. fusiformis, also amplified with these markers. Conclusions: The SSR markers developed in this study will be helpful in defining the population structure and genetics of Claviceps strains. They will also provide valuable tools for plant breeders needing to identify resistance in crops or for researchers examining fungal movements across environments. © The Author(s).

引用

页码：1 / 13

页数：12

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