Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter

被引：31

作者：

Park S.J. ^{[1
]}

Park J.P. ^{[1
]}

Lee S.Y. ^{[1
]}

机构：

[1] Biomolecular Eng. Natl. Res. Lab., Department of Chemical Engineering, Korea Advanced Inst. Sci./Technol., Yusong-gu, Taejon 305-701

来源：

Biotechnology Letters | 2002年 / 24卷 / 3期

关键词：

Pilot-scale fermenter; Poly(3-hydroxybutyrate); Recombinant E. coli; Whey;

D O I：

10.1023/A:1014196906095

中图分类号：

学科分类号：

摘要：

Production of poly(3-hydroxybutyrate) [P(3HB)] from whey by fed-batch culture of recombinant Escherichia coli CGSC 4401 harboring a plasmid containing the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes was examined in a 30 1 fermenter supplying air only. With lactose below 2 g 1-1, cells grew to 12 g dry cell 1-1 with 9% (w/w) P(3HB) content. Accumulation of P(3HB) could be triggered by increasing lactose to 20 g 1-1. By employing this strategy, 51 g dry cell 1-1 was obtained with a 70% (w/w) P(3HB) content after 26 h. The productivity was 1.35 g P(3HB) 1-1 h-1. The same fermentation strategy was used in a 300 1 fermenter, and 30 g dry cell 1-1 with 67% (w/w) P(3HB) content was obtained in 20 h.

引用

页码：185 / 189

页数：4

共 16 条

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