Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-mediated Isothermal Amplification Method

被引：0
作者：
Xiaoyan Guan
Jinchao Guo
Ping Shen
Litao Yang
Dabing Zhang
机构：
[1] Shanghai Jiao Tong University,GMO Detection Laboratory, SJTU
[2] Ministry of Agriculture,Bor Luh Food Safety Center, School of life Science and Biotechnology
[3] Shanghai Jiao Tong University,Development Center for Science and Technology
来源：
Food Analytical Methods | 2010年 / 3卷
关键词：
Loop-mediated Isothermal Amplification; Visual Detection; Genetically Modified Soybean;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To develop effective alternatives for detecting genetically modified organisms (GMOs), we reported one optimized visual loop-mediated isothermal amplification (LAMP) method for the detection of exogenous DNA targets from two GM soybean events in this study. This isothermal amplification can be performed within 40 min without polymerase chain reaction (PCR) equipment and the derived LAMP products can be directly observed by naked eye employing SybrGreen I dye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about 4 copies of haploid soybean genomic DNA, and which were much higher than those of reported conventional PCR assays. Furthermore, the high specificity of LAMP assays was determined. All the results demonstrated that the developed visual LAMP assays are convenient, cost-efficient, and rapid for on spot detection of GMOs.
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页码：313 / 320
页数：7
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