Long Wavelength Fluorescence Ratiometric Zinc Biosensor

被引：0
|
作者：
Hui Hui Zeng
Evgenia G. Matveeva
Andrea K. Stoddard
Carol A. Fierke
Richard B. Thompson
机构：
[1] University of Maryland School of Medicine,Department of Biochemistry and Molecular Biology
[2] University of Michigan,Department of Chemistry
来源：
Journal of Fluorescence | 2013年 / 23卷
关键词：
Zinc ion; Fluorescence biosensor; Ratiometric; Carbonic anhydrase; FRET; Forster resonance energy transfer;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A protein-based emission ratiometric fluorescence biosensor is described that exhibits sensitivity to free zinc ion in solution down to picomolar concentrations. Ratiometric measurements are widely used to assure accurate quantitation, and emission ratios are preferred for laser scanning microscopes such as confocal fluorescence microscopes. The relatively long emission wavelengths used are well suited to studies in tissues and other matrices which exhibit significant fluorescence background, and the apo-carbonic anhydrase moiety recognizes zinc ion with high and controllable specificity.
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页码：375 / 379
页数：4
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