Aberrant expression and function of TCF4 in the proliferation of hepatocellular carcinoma cell line BEL-7402

被引：0
作者：
Dong Hong ZHAO
Jian Jun HONG
Shi Ying GUO
Run Lin YANG
Jun YUAN
Chuan Jun WEN
Kai Ya ZHOU
Chao Jun LI
机构：
[1] The Jiangsu Key Laboratory of Molecular Medical Biotechnology,
[2] College of Life Science,undefined
[3] Nanjing Normal University,undefined
来源：
Cell Research | 2004年 / 14卷
关键词：
Wnt signaling pathway; β-catenin; dominant negative TCF4;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Wnt signaling pathway is essential for development and tumorigenesis, however, this signaling pathway in the progress of hepatocellular carcinoma (HCC) remains unclear. In this paper, we studied the function of human T-cell transcription factor-4 (TCF4), a key factor of Wnt signaling pathway, on the proliferation of HCC cell line. We showed that the expression of TCF4 mRNA in HCC cell line BEL-7402 was higher than that in immortalized normal liver cell line L02. Blockage of Wnt pathway by ΔNTCF4, a dominant negative TCF4, could suppress BEL-7402 cells growth and decrease the expression of cyclin D1 and c-myc, two of target genes of Wnt pathway. On the other hand, stimulating Wnt pathway by introducing a degradation-resistant β-catenin S37A could increase BEL-7402 cells proliferation. But all the treatments had no effect on L02 cells. Our data indicated that TCF4 might be another key factor in Wnt pathway involved in HCC cells proliferation and TCF4 could be an effective therapeutic target for suppressing the growth of hepatocellular cancers.
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页码：74 / 80
页数：6
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