Expression of a CaMV::sak-gusA-mgfp gene in tobacco plants

被引：0
作者：
Anna Dobrowolska
Andrzej K. Kononowicz
机构：
[1] University of Lodz,Department of Cytogenetics and Plant Molecular Biology
来源：
World Journal of Microbiology and Biotechnology | 2004年 / 20卷
关键词：
recombinant proteins; staphylokinase; transgenic plants;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA1303. The presence of a CaMV::sak-gusA-mgfp gene in Agrobacterium was confirmed by polymerase chain reaction PCR. Tobacco seedlings were used as explants for Agrobacterium tumefaciens-mediated transformation with the pCAMBIA1303sak vector carrying the fusion gene construct CaMV::sak-gusA-mgfp and the expression of the fusion gene was identified in Nicotiana tabacum plants by β-glucuronidas assay.
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页码：333 / 335
页数：2
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