Recombinant expression of human transforming growth factor-β isoforms in Chinese hamster ovary cells

被引：0
作者：
B. Ramani
Paturu Kondaiah
机构：
[1] Indian Institute of Science,Department of Molecular Reproduction, Development and Genetics
来源：
Journal of Biosciences | 1998年 / 23卷
关键词：
Recombinant DNA; gene expression; growth factors; TGF-; CHO cells;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Transforming growth factor-βs (TGF-βs) are multi functional growth modulators implicated in several physiological processes which include embryogenesis, inflammation, immune-suppression, wound healing, carcinogenesis and cellular differentiation. For clinical use, recombinant expression of TGF-βs is the only practical source because of very low yields from natural sources. Here, we report the recombinant expression of human TGF-βl and TGF-β2 in a mammalian expression system using a high expression eukaryotic vector driven by a cytomegalovirus promoter. Expression levels are as high as 0.97 μg/ml of TGF-βl and 0.24 μg/ml of TGF-β2 in conditioned media, sufficient for purification without the need for amplification of the gene using methotrexate.
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页码：577 / 583
页数：6
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