Detection and determination of some phenolic and cinnamic acids in plant extracts

被引:0
作者
N. Yu. Sipkina
Yu. A. Skorik
机构
[1] St. Petersburg State Chemical Pharmaceutical Academy,Institute of High Molecular Compounds
[2] Russian Academy of Sciences,undefined
来源
Journal of Analytical Chemistry | 2015年 / 70卷
关键词
phenolic acids; cinnamic acids; HPLC; plant extracts;
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摘要
Chromatographic conditions were optimized and performance characteristics were evaluated for a procedure for the detection and determination of 12 phenolic and cinnamic acids in plant extracts by HPLC with spectrophotometric detection. Chromatographic separation was performed in the gradient elution mode, using an aqueous solution of trifluoroacetic acid and acetonitrile as a mobile phase. Sensitivity was increased by performing spectrophotometric detection at the absorbance maxima of individual acids. The procedure was used for the detection and determination of phenolic content in dried extracts from Astragalus dolichophyllus and Astragalus brachylobus.
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页码:1406 / 1411
页数:5
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