Palm is expressed in both developing and adult mouse lens and retina

被引：8
作者：
Castellini M. [1 ,4 ]
Wolf L.V. [2 ]
Chauhan B.K. [2 ,5 ]
Galileo D.S. [1 ]
Kilimann M.W. [3 ]
Cvekl A. [2 ]
Duncan M.K. [1 ]
机构：
[1] Department of Biological Sciences, University of Delaware, Newark
[2] Depts. of Ophthalmology and Visual Sciences and Molecular Genetics, Albert Einstein College of Medicine, Bronx
[3] Department of Cell and Molecular Biology, Uppsala University
[4] Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia
[5] Developmental Biology Division, Department of Ophthalmology, Children's Hospital Research Foundation, Cincinnati, OH 45229
来源：
BMC Ophthalmology | / 5卷 / 1期
关键词：
Neural Retina; Retinal Pigment Epithelium; Pax6 Expression; Optic Vesicle; Lens Fiber Cell;
D O I：
10.1186/1471-2415-5-14
中图分类号：
学科分类号：
摘要：
Background: Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Earlier studies have shown brain preferred Palm expression, although this protein is a major water insoluble protein in chicken lens fiber cells and the Palm gene may be regulated by Pax6. Methods: The expression profile of Palm protein in the embryonic, newborn and adult mouse eye as well as dissociated retinal neurons was determined by confocal immunofluorescence. The relative mRNA levels of Palm, Palmdelphin (PalmD) and paralemmin2 (Palm2) in the lens and retina were determined by real time rt-PCR. Results: In the lens, Palm is already expressed at 9.5 dpc in the lens placode, and this expression is maintained in the lens vesicle throughout the formation of the adult lens. Palm is largely absent from the optic vesicle but is detectable at 10.5 dpc in the optic cup. In the developing retina, Palm expression transiently upregulates during the formation of optic nerve as well as in the formation of both the inner and outer plexiform layers. In short term dissociated chick retinal cultures, Palm protein is easily detectable, but the levels appear to reduce sharply as the cultures age. Palm mRNA was found at much higher levels relative to Palm2 or PalmD in both the retina and lens. Conclusion: Palm is the major paralemmin family member expressed in the retina and lens and its expression in the retina transiently upregulates during active neurite outgrowth. The expression pattern of Palm in the eye is consistent with it being a Pax6 responsive gene. Since Palm is known to be able to drive membrane formation in brain neurons, it is possible that this molecule is crucial for the increase in membrane formation during lens fiber cell differentiation. © 2005 Castellini et al; licensee BioMed Central Ltd.
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  • [1] Chow R.L., Lang R.A., Early eye development in vertebrates, Annu Rev Cell Dev Biol, 17, pp. 255-296, (2001)
  • [2] Ochi H., Sakagami K., Ishii A., Morita N., Nishiuchi M., Ogino H., Yasuda K., Temporal expression of L-Maf and RaxL in developing chicken retina are arranged into mosaic pattern, Gene Expr Patterns, 4, 5, pp. 489-494, (2004)
  • [3] Lecoin L., Sii-Felice K., Pouponnot C., Eychene A., Felder-Schmittbuhl M.P., Comparison of maf gene expression patterns during chick embryo development, Gene Expr Patterns, 4, 1, pp. 35-46, (2004)
  • [4] Collinson J.M., Quinn J.C., Hill R.E., West J.D., The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo, Dev Biol, 255, 2, pp. 303-312, (2003)
  • [5] Dyer M.A., Livesey F.J., Cepko C.L., Oliver G., Prox1 function controls progenitor cell proliferation and horizontal cell genesis in the mammalian retina, Nat Genet, 34, 1, pp. 53-58, (2003)
  • [6] Duncan M.K., Cui W., Oh D.J., Tomarev S.I., Prox1 is differentially localized during lens development, Mech Dev, 112, pp. 195-198, (2002)
  • [7] Li X., Ma W., Barker J.L., Piatigorsky J., Transient expression of glutamate decarboxylase and gamma-amino butyric acid in embryonic lens fibers of the rat, Dev Dyn, 203, 4, pp. 448-455, (1995)
  • [8] Giger R.J., Wolfer D.P., De Wit G.M., Verhaagen J., Anatomy of rat semaphorin III/collapsin-1 mRNA expression and relationship to developing nerve tracts during neuroembryogenesis, J Comp Neurol, 375, 3, pp. 378-392, (1996)
  • [9] Baechner D., Liehr T., Hameister H., Altenberger H., Grehl H., Suter U., Rautenstrauss B., Widespread expression of the peripheral myelin protein-22 gene (PMP22) in neural and non-neural tissues during murine development, J Neurosci Res, 42, 6, pp. 733-741, (1995)
  • [10] Claudio J.O., Lutchman M., Rouleau G.A., Widespread but cell type-specific expression of the mouse neurofibromatosis type 2 gene, Neuroreport, 6, 14, pp. 1942-1946, (1995)
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