Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq

被引：0
作者：
David Redmond
Asaf Poran
Olivier Elemento
机构：
[1] Tri-Institutional Training Program in Computational Biology and Medicine,Institute for Computational Biomedicine & Institute for Precision Medicine
[2] Weill Cornell Medicine,undefined
来源：
Genome Medicine | / 8卷
关键词：
Read Length; Alpha Chain; Beta Chain; Jurkat Cell Line; TRBV Gene;
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摘要：
Accurate characterization of the repertoire of the T-cell receptor (TCR) alpha and beta chains is critical to understanding adaptive immunity. Such characterization has many applications across such fields as vaccine development and response, clone-tracking in cancer, and immunotherapy. Here we present a new methodology called single-cell TCRseq (scTCRseq) for the identification and assembly of full-length rearranged V(D)J T-cell receptor sequences from paired-end single-cell RNA sequencing reads. The method allows accurate identification of the V(D)J rearrangements for each individual T-cell and has the novel ability to recover paired alpha and beta segments. Source code is available at https://github.com/ElementoLab/scTCRseq.
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