Comparative anatomy of embryogenic and non-embryogenic calli frompimpinella brachycarpa

被引:0
作者
Haeyoung Na
Ki Woo Kim
Yurina Kwack
Sung Kyeom Kim
Changhoo Chun
机构
[1] Seoul National University,Research Institute for Agriculture and Life Sciences
[2] Seoul National University,Department of Plant Science
[3] Seoul National University,National Instrumentation Center for Environmental Management
来源
Journal of Plant Biology | 2007年 / 50卷
关键词
callus; differentiation; electron microscopy; embryo; regeneration;
D O I
暂无
中图分类号
学科分类号
摘要
Anatomical differences between embryogenic and non-embryogenic calli ofPimpinella brachycarpa were investigated by light microscopy and electron microscopy. Initial callus tissue emerged from expiants after 14 d of culturing. The embryogenie calli (EC) were firm, rather opaque, and light yellow in color. The cells usually formed small, compact clusters. Nonembryogenic calli (NEC), however, were friable, semitransparent, and yellow or gray. These formed relatively larger and loosely held clusters. Scanning electron microscopy showed that EC were composed of individual compact and spherical cells that were rather regular in size and approximately 20 µm long. All were tightly held together and appeared to organize globular embryos. In contrast, the NEC comprised elongated and loosely held cells that were approximately 50 µm long. Tubular and u-shaped NEC cells protruded irregularly, and were of varying heights along the cell aggregates. Transmission electron microscopy of the EC revealed typical eukaryotic cytoplasmic components, including nuclei, mitochondria, and vacuoles in the cytoplasm enclosed by an electron-transparent cell wall. Based on the numerous ribosomes within the cytoplasm, these cells appeared to be well-organized and metabolically active. The NEC cells were much larger and more highly vacuolated than those of the EC. In ultrathin sections, the former seemed to be almost devoid of other cellular contents except for plastids and nuclei. Furthermore, EC and NEC showed different regeneration capacities in their somatic embryo formation. Most EC produced hyperhydric somatic embryos, followed by normal somatic embryos; whereas only a few shooted or rooted somatic embryos arose from the NEC.
引用
收藏
页码:344 / 350
页数:6
相关论文
共 33 条
[21]   High levels of non-methylesterified pectins and low levels of peripherally located pectins in loosely attached non-embryogenic callus of carrot [J].
Iwai, H ;
Kikuchi, A ;
Kobayashi, T ;
Kamada, H ;
Satoh, S .
PLANT CELL REPORTS, 1999, 18 (7-8) :561-566
[22]   Activity of glutathione S-transferase toward some herbicides and its regulation by benoxacor in non-embryogenic callus and in vitro regenerated tissues of Zea mays [J].
Del Buono, Daniele ;
Michell, Maurizio ;
Scarponi, Luciano ;
Standardi, Alvaro .
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2006, 85 (02) :61-67
[23]   Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton via Efficient Selection and Timely Subculture of Somatic Embryos [J].
Wu, Shen-Jie ;
Wang, Hai-Hai ;
Li, Fei-Fei ;
Chen, Tian-Zi ;
Zhang, Jie ;
Jiang, Yan-Jie ;
Ding, Yezhang ;
Guo, Wang-Zhen ;
Zhang, Tian-Zhen .
PLANT MOLECULAR BIOLOGY REPORTER, 2008, 26 (03) :174-185
[24]   Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton via Efficient Selection and Timely Subculture of Somatic Embryos [J].
Shen-Jie Wu ;
Hai-Hai Wang ;
Fei-Fei Li ;
Tian-Zi Chen ;
Jie Zhang ;
Yan-Jie Jiang ;
Yezhang Ding ;
Wang-Zhen Guo ;
Tian-Zhen Zhang .
Plant Molecular Biology Reporter, 2008, 26 :174-185
[25]   Agrobacterium-mediated transformation using embryogenic calli in Satsuma mandarin (Citrus unshiu Marc.) cv. Miyagawa wase [J].
Md. Adnan Al Bachchu ;
Seong Beom Jin ;
Jeong Won Park ;
Hyeon Jin Sun ;
Su Hyun Yun ;
Hyo Yeon Lee ;
Dong Sun Lee ;
Quan Chun Hong ;
Yong Woo Kim ;
Key Zung Riu ;
Jae Hoon Kim .
Horticulture, Environment, and Biotechnology, 2011, 52 :170-175
[26]   Agrobacterium-mediated transformation using embryogenic calli in Satsuma mandarin (Citrus unshiu Marc.) cv. Miyagawa wase [J].
Al Bachchu, Md Adnan ;
Jin, Seong Beom ;
Park, Jeong Won ;
Sun, Hyeon Jin ;
Yun, Su Hyun ;
Lee, Hyo Yeon ;
Lee, Dong Sun ;
Hong, Quan Chun ;
Kim, Yong Woo ;
Riu, Key Zung ;
Kim, Jae Hoon .
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, 2011, 52 (02) :170-175
[27]   Production of non-embryogenic synthetic seeds for short-term cold storage and ISSR marker-assisted genetic fidelity analysis of Coleus forskohlii (Willd) Briq. plants [J].
Badhepuri, Mahesh Kumar ;
Aileni, Mahender ;
Beeravelli, Prabhakar Rao ;
Durgam, Sathish Kumar ;
Sandhya, Dulam ;
Shekhawat, Mahipal S. ;
Singisala, Nageswara Rao .
PLANT CELL TISSUE AND ORGAN CULTURE, 2025, 160 (02)
[28]   Comparative Analysis of Sterol Composition of Embryogenic and Nonembryogenic Cell Lines of Larix sibirica Ledeb. [J].
Semenova, N. V. ;
Shmakov, V. N. ;
Konstantinov, Yu. M. ;
Dudareva, L. V. .
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, 2023, 70 (02)
[29]   Comparative Transcriptomic Insights into the Mechanisms Underlying Maize (Zea mays L.) Embryogenic Callus Differentiation [J].
Dai, Liqiang ;
Li, Tianjiao .
AGRONOMY-BASEL, 2024, 14 (08)
[30]   A rapid and efficient protocol for genotype-independent, Agrobacterium-mediated transformation of indica and japonica rice using mature seed-derived embryogenic calli [J].
Jain, Nitin ;
Khurana, Paramjit ;
Khurana, Jitendra P. .
PLANT CELL TISSUE AND ORGAN CULTURE, 2022, 151 (01) :59-73