Sex differences in p-aminohippuric acid transport in rat kidney: Role of membrane fluidity and expression of OAT1

被引：0
作者：
Jorgelina A. Cerrutti
Anabel Brandoni
Nora B. Quaglia
Adriana M. Torres
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来源：
Molecular and Cellular Biochemistry | 2002年 / 233卷
关键词：
organic anion transporter; p-aminohippuric acid; membrane fluidity; brush border membrane vesicles; basolateral membrane vesicles; renal tubular secretion;
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摘要：
Sex differences in the kinetic parameters of p-aminohippuric acid (PAH) transport in brush border (BBMV) and basolateral (BLMV) membrane vesicles from kidney cortex have been observed. Membrane fluidity of BBMV was higher in females as compared with male rats as indicated by anisotropy values (0.1897 ± 0.0010 vs. 0.2003 ± 0.0014, p < 0.05, for females and males respectively). Membrane fluidity of BLMV were similar in both sexes. Western blot studies revealed that OAT1 protein in female BLMV was present at only 40% of level found in BLMV from male rats. The lower expression of OAT1 in BLMV in association with the higher BBMV fluidity (which may affect the affinity of PAH transporter in this membrane domain) observed in females may be responsible, at least in part, for the gender difference described in renal PAH secretion.
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页码：175 / 179
页数：4
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  • [1] Burckhardt G(2000)Structure of renal organic anion and cation transporters Am J Physiol 278 F853-F866
  • [2] Wolff N(2000)Cellular and molecular aspects of drug transport in the kidney Kidney Int 58 944-958
  • [3] Inui K-I(2000)Molecular pharmacology of organic anion transporters Am J Physiol 279 F216-F232
  • [4] Masuda S(2001)Regulation of renal tubular secretion of organic compounds Kidney Int 59 17-30
  • [5] Saito H(1997)Expression cloning and characterization of a novel multispecific organic anion transporter J Biol Chem 272 18526-18529
  • [6] Van Aubel R(1997)Expression cloning and characterization of ROAT1: The basolateral organic anion transporter in rat kidney J Biol Chem 272 30088-30095
  • [7] Masereeuw R(2000)Multidrug resistance protein Mrp2 mediates ATP-dependent transport of classical renal organic anion p-aminohippurate Am J Physiol 279 F713-F717
  • [8] Russel FGM(2000)ATP-dependent paraaminohippurate transport by apical multidrug resistance protein MRP2 Kidney Int 57 1636-1642
  • [9] Berkhin EB(1972)Sex differences in organic ion transport by rat kidney Proc Soc Exp Biol Med 141 258-262
  • [10] Humphreys MH(1993)Postnatal development of sex differences in renal tubular transport of p-aminohippurate (PAH) in rats Exp Toxic Pathol 45 309-313
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