Isolation and investigation of the ethylene-producing enzyme of cotton

被引：0
作者：
E. M. Sultanova
O. N. Veshkurova
A. S. Takanaev
Ya. S. Ziyaev
E. A. Il'yasov
Sh. I. Salikhov
机构：
[1] Academy of Sciences of the Republic of Uzbekistan,A. S. Sadykov Institute of Bioorganic Chemistry
[2] Academy of Sciences of the Republic of Uzbekistan,Institute of the Chemistry of Plant Substances
来源：
Chemistry of Natural Compounds | 1999年 / 35卷
关键词：
Ethylene; Organic Chemistry; Chromatographic Method; Ethylene Biosynthesis; Differential Centrifugation;
D O I：
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中图分类号：
学科分类号：
摘要：
The activity of the ethylene-producing enzyme (EPE) in a homogenate of cotton leaves and in fractions obtained via differential centrifugation and fractional precipitation is studied. A model system is developed for testing EPE. The effect of known activators and inhibitors of ethylene biosynthesis on the EPE activity is studied. Chromatographic methods are used to prepare the highly purified enzyme from the water-soluble fraction of cotton proteins and to identify it as ACC-oxidase. The enzyme has MM ∼38 kDa and converts aminocyclopropanecarboxylic acid (ACC) into ethylene.
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页码：567 / 569
页数：2
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