Vector competence of three species of mosquitoes to Ingwavuma virus (Manzanilla orthobunyavirus), a new bunyavirus found circulating in India

被引：2
作者：
Kumar S. [1 ]
Sreelekshmi P.R. [1 ]
Godke Y.S. [1 ]
Sudeep A.B. [1 ]
机构：
[1] Division of Medical Entomology, ICMR-National Institute of Virology, (Indian Council of Medical Research), Microbial Containment Complex, 130/1, Sus Road, Pashan, Pune
来源：
VirusDisease | 2023年 / 34卷 / 1期
基金：
美国国家科学基金会;
关键词：
Aedes aegypti; Culex quinquefasciatus; Culex tritaeniorhynchus; Ingwavuma virus; Manzanilla orthobunyavirus; Vector competence;
D O I：
10.1007/s13337-023-00808-z
中图分类号：
学科分类号：
摘要：
Ingwavuma virus (INGV), a mosquito-borne arbovirus reported from Africa and Southeast Asia has been found circulating in India as evidenced by virus isolation and antibody prevalence. INGV is now classified as Manzanilla orthobunyavirus belonging to family Peribunyaviridae. The virus is maintained in nature in a pig-mosquito-bird cycle. Human infection has been confirmed by virus isolation and detection of neutralizing antibodies. A study was initiated to determine the vector competence of Aedes aegypti, Culex quinquefasciatus, and Cx tritaeniorhynchus mosquitoes to INGV due to their high prevalence in India. Mosquitoes were oral fed on viraemic mice and INGV dissemination to legs, wings, salivary glands (saliva) was studied alongwith virus growth kinetics. The three mosquitoes replicated INGV with maximum titers of 3.7, 3.7 and 4.7log10TCID50/ml respectively and maintained the virus till 16th day post infection. However, vector competence and horizontal transmission to infant mouse was demonstrated only by Cx quinquefasciatus mosquitoes. Vertical or trans-ovarial transmission of INGV could not be demonstrated in the mosquito during the study. Though no major outbreak involving humans has been reported yet, the potential of the virus to replicate in different species of mosquitoes and vertebrates including humans pose a threat to public health should there be a change in its genome. © 2023, The Author(s), under exclusive licence to Indian Virological Society.
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页码：15 / 20
页数：5
相关论文
共 24 条
[1]  
McIntosh B.M., McGillivray G.M., Dickinson D.B., Ingwavuma virus: an arbovirus isolated in South Africa, S Afr J Med Sci, 30, 3, pp. 67-70, (1965)
[2]  
Causey O.R., Kemp G.E., Causey C.E., Lee V.H., Isolations of Simbu-group viruses in Ibadan, Nigeria 1964–69, including the new types Sango, Shamonda, Sabo and Shuni, Ann Trop Med Parasitol, 66, 3, pp. 357-362, (1972)
[3]  
Top F.H., Kraivapan C., Grossman R.A., Rozmiarek H., Edelman R., Gould D.J., Ingwavuma virus in Thailand: infection of domestic pigs, Am J Trop Med Hyg, 23, pp. 251-257, (1974)
[4]  
Converse J.D., Tan R.I., Rachman I.T., Lee V.H., Shope R.E., Ingwavuma virus (Simbu group) from Culex and Mansonia mosquitoes (Diptera: Culicidae) in Indonesia, J Med Entomol, 22, pp. 339-342, (1985)
[5]  
Fagbami A.H., Fabiyi A., Arbovirus studies in two towns in western state of Nigeria, J Trop Geogr Med, 27, 1, pp. 59-62, (1975)
[6]  
Fagbami A., Human arthropod-borne virus infections in Nigeria. Serological and virological investigations and Shaki, Oyo State, J Hyg Epidemiol Microbiol Immunol, 22, pp. 184-189, (1978)
[7]  
Pavri K.M., Sheikh B.H., Singh K.R.P., Rajagopalan P.K., Casals J., Balagodu virus, a new arbovirus isolated from Ardeola grayii (Sykes) in Mysore state, South India, Indian J Med Res, 57, pp. 758-764, (1969)
[8]  
Ladner J.T., Savji N., Lofts L., Et al., Genomic and phylogenetic characterization of viruses included in the Manzanilla and Oropouche species complexes of the genus Orthobunyavirus, family Bunyaviridae, J Gen Virol, 95, 5, pp. 1055-1066, (2014)
[9]  
Whitmer S.L.M., Yadav P.D., Sarkale P., Et al., Characterization of unknown orthobunya-like viruses from India, Viruses, 10, 9, (2018)
[10]  
Sudeep A.B., Shaikh N., Ghodke Y.S., Ingale V.S., Gokhale M.D., Vector competence of certain Culex and Aedes mosquitoes for the Chittoor virus, the Indian variant of the Batai virus, Can J Microbiol, 64, 8, pp. 581-588, (2018)
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