Mg2+-dependent folding of a large ribozyme without kinetic traps

被引:0
作者
Xing-wang Fang
Tao Pan
Tobin R. Sosnick
机构
[1] University of Chicago,Department of Biochemistry and Molecular Biology
来源
Nature Structural Biology | 1999年 / 6卷
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摘要
The folding kinetics of the catalytic domain of Bacillus subtilis ribonuclease P is analyzed here by fluorescence and catalytic activity. The folding pathway is apparently free of kinetic traps, as indicated by a decrease in folding rates upon the addition of urea. We apply Mg2+ and urea chevron analysis to fully describe the folding and unfolding kinetics of this ribozyme. A folding scheme containing two kinetic intermediates completely accounts for the free energy, the Mg2+ Hill coefficient and the surface buried in the equilibrium transition. At saturating Mg2+concentrations, folding is limited by a barrier that is independent of Mg2+ and urea. These results describe the first trap-free folding pathway of a large ribozyme and indicate that kinetic traps are not an obligate feature of RNA folding.
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页码:1091 / 1095
页数:4
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