Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS

被引：11

作者：

Pan C.-X. ^{[1
]}

Xu X.-Z. ^{[1
]}

He H.-M. ^{[1
]}

Cai X.-J. ^{[1
]}

Zhang X.-J. ^{[1
]}

机构：

[1] Dept. of Chem., Zhejiang Univ.

来源：

Journal of Zhejiang University-SCIENCE B | 2005年 / 6卷 / 1期

关键词：

(S; S)-Whelk-O; 1; 2-butene-1,4-diol; ChiraSpher; Isomers separation; Lafutidine; LC-MS;

D O I：

10.1631/jzus.2005.B0074

中图分类号：

学科分类号：

摘要：

The cis and trans isomers separation of 2-butene-1,4-diol and lafutidine was studied by HPLC (high performance liquid chromatography) on two kinds of chiral columns: (S, S)-Whelk-O 1 and ChiraSpher. The isomers of 2-butene-1,4-diol can be separated on both chiral columns while the isomers of lafutidine can only be resolved on ChiraSpher column. The influence of different type and amount of mobile phase modifier on the isomers separation was studied. The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61 on (S, S)-Whelk-O 1 column with hexane-ethanol (97:3, v/v) as the mobile phase. The resolution of lafutidine was 1.89 on ChiraSpher column with hexane-ethanol-THF-diethylamine (92:3:5:0.1, v/v/v/v) as the mobile phase. LC-MS (mass spectrometry) methods were developed to identify the isomer peaks.

引用

页码：74 / 78

页数：4

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