Expression of the inhibin/activin subunits alpha (α), beta-A (βA) and beta-B (βB) in benign human endometrial polyps and tamoxifen-associated polyps

被引:23
作者
Mylonas I. [1 ,2 ]
Makovitzky J. [2 ,3 ]
Fernow A. [2 ,3 ]
Richter D.-U. [2 ,3 ]
Jeschke U. [1 ]
Briese V. [2 ,3 ]
Gerber B. [1 ]
Friese K. [1 ]
机构
[1] First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, 80337 Munich
[2] University of Rostock, Rostock
[3] Department of Obstetrics and Gynaecology, University of Rostock, Rostock
关键词
Endometrial polyps; Endometrium; Immunohistochemistry; Inhibin/activin subunits; Tamoxifen;
D O I
10.1007/s00404-004-0666-2
中图分类号
学科分类号
摘要
Background: Inhibins (INH) are dimeric glycoproteins, composed of an alpha subunit (INH-α) and one of two possible beta subunits (INH-βA or INH-βB). They have substantial roles in human reproduction and in endocrine-responsive tumours. Therefore, the aims of this study were to determine the frequency and tissue distribution of INH-α, INH-βA and INH-βB in normal human endometrium and glandular-cystic endometrial polyps, and polyps caused by tamoxifen use. Materials and methods: Tissue samples were obtained from women in the proliferative, early secretory and late secretory phase as well as glandular-cystic polyps and endometrial polyps associated with tamoxifen use (n=5 each). Immunohistochemistry with specific monoclonal antibodies, a semi-quantitative analysis and statistical evaluation was performed. Results: INH-α, INH-βA and INH-βB were primarily observed in glandular and luminal epithelial cells, with a variant staining intensity in stromal cells. INH-α in glands was significantly higher during the early secretory phase (p<0.05) and the late secretory phase (p<0.01) than in the proliferative phase with a significant difference between the early secretory and the late secretory phases (p<0.01). INH-βA expression was significantly higher during the late secretory than the proliferative phase (p<0.05) and the late secretory than the early secretory phase (p<0.05), with no significant differences for INH-βB. Glandular-cystic polyps showed significantly lower expression of INH-α and INH-βA than the late secretory endometria (p<0.05 and p<0.01 respectively). Additionally, tamoxifen-associated polyps also demonstrated a significantly lower expression of INH-α and INH-βA than late secretory endometria (p<0.01 and p<0.01 respectively). No statistical differences were observed between tamoxifen-associated and glandular-cystic polyps. Discussion: INH-α, INH-βA and INH-βB were expressed in normal endometrium and endometrial polyps. A cyclical expression of INH-α and INH-βA in normal glands may reflect a functional and hormone-dependent role in human endometrium. Significant differences in staining reaction between the late secretory endometria and polyps suggest that this tissue remains in the proliferating state rather than the secretory state. Therefore, endometrial polyps may be tumours of dysregulation with mainly proliferating characteristics, being unable to synchronise with normal endometrium. © Springer-Verlag 2004.
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页码:59 / 66
页数:7
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