Gene expression profiling of bovine in vitro adipogenesis using a cDNA microarray

被引：45
作者：
Tan S.H. [1 ,2 ]
Reverter A. [1 ]
Wang Y.H. [1 ]
Byrne K.A. [1 ]
McWilliam S.M. [1 ]
Lehnert S.A. [1 ]
机构：
[1] The Cooperative Research Center for Cattle and Beef Quality, CSIRO Livestock Industries, Queensland Bioscience Precinct, St. Lucia, QLD 4067
[2] The Cooperative Research Center for Cattle and Beef Quality, Faculty of the Sciences, University of New England, Armidale
来源：
Functional & Integrative Genomics | 2006年 / 6卷 / 3期
关键词：
Adipogenesis; Bovine; cDNA microarray; Gene expression;
D O I：
10.1007/s10142-005-0016-x
中图分类号：
学科分类号：
摘要：
The gene expression profile of bovine bone marrow stromal cells undergoing adipogenesis was established using a custom cDNA microarray. Cells that were treated with adipogenic stimulants and those that were not were collected at each of the six time points, and gene expression differences between the treated and untreated samples within each time point were compared using a microarray. Statistical analyses revealed that 158 genes showed a minimum fold change of 2 in at least one of the five post-differentiation time points. These genes are involved in various cellular pathways and functions, including lipogenesis, glycolysis, cytoskeleton remodelling, extracellular matrix, transcription as well as various signalling pathways such as insulin, calcium and wingless signalling. The experiment also identified 17 differentially expressed (DE) microarray elements with no assigned function. Quantitative real-time PCR was employed to validate eight DE genes, and the PCR data were found to reproduce the microarray data for these eight genes. Subsequent gene ontology annotation was able to provide a global overview of the molecular function of DE genes during adipogenesis. This analysis was able to indicate the importance of different gene categories at various stages of adipogenic conversion, thereby providing further insights into the molecular changes during bovine adipogenesis. © Springer-Verlag 2006.
引用
收藏
页码：235 / 249
页数：14
相关论文
共 50 条
[1]  
Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J., Basic local alignment search tool, J Mol Biol, 215, pp. 403-410, (1990)
[2]  
Ashburner M., Ball C.A., Blake J.A., Botstein D., Butler H., Cherry J.M., Davis A.P., Dolinski K., Dwight S.S., Eppig J.T., Harris M.A., Hill D.P., Issel-Tarver L., Kasarskis A., Lewis S., Matese J.C., Richardson J.E., Ringwald M., Rubin G.M., Sherlock G., Gene ontology: Tool for the unification of biology, Nat Genet, 25, pp. 25-29, (2000)
[3]  
Batchvarova N., Wang X.Z., Ron D., Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153), EMBO J, 14, pp. 4654-4661, (1995)
[4]  
Burton G.R., Nagarajan R., Peterson C.A., McGehee Jr. R.E., Microarray analysis of differentiation-specific gene expression during 3T3-L1 adipogenesis, Gene, 329, pp. 167-185, (2004)
[5]  
Camp H.S., Tafuri S.R., Leff T., c-Jun N-terminal kinase phosphorylates peroxisome proliferator-activated receptor-gamma1 and negatively regulates its transcriptional activity, Endocrinology, 140, pp. 392-397, (1999)
[6]  
Claussen M., Kubler B., Wendland M., Neifer K., Schmidt B., Zapf J., Braulke T., Proteolysis of insulin-like growth factors (IGF) and IGF binding proteins by cathepsin D, Endocrinology, 138, pp. 3797-3803, (1997)
[7]  
Glare E.M., Divjak M., Bailey M.J., Walters E.H., beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalizing mRNA levels, Thorax, 57, pp. 765-770, (2002)
[8]  
Groeneveld E., Garcia-Cortes L.A., VCE 4.0, a (co)variance components package for frequentists and Bayesians, Proc 6th World Congr Genet Livest Prod, 27, pp. 455-458, (1998)
[9]  
Hamalainen H.K., Tubman J.C., Vikman S., Kyrola T., Ylikoski E., Warrington J.A., Lahesmaa R., Identification and validation of endogenous reference genes for expression profiling of T helper cell differentiation by quantitative real-time RT-PCR, Anal Biochem, 299, pp. 63-70, (2001)
[10]  
Hansen C., Fu A., Li C., Dixon W.T., Christopherson R., Moore S.S., Global gene expression patterns spanning 3T3-L1 preadipocyte differentiation, Can J Anim Sci, 84, pp. 367-376, (2004)
12345